Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLKML_RS10010 Genome accession   NZ_CP178628
Coordinates   555518..556558 (-) Length   346 a.a.
NCBI ID   WP_413285221.1    Uniprot ID   -
Organism   Vibrio sp. MA40-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550518..561558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKML_RS09980 (ACLKML_09980) metK 550774..551925 (+) 1152 WP_413285219.1 methionine adenosyltransferase -
  ACLKML_RS09985 (ACLKML_09985) - 552087..552590 (+) 504 WP_413285864.1 SprT family zinc-dependent metalloprotease -
  ACLKML_RS09990 (ACLKML_09990) rsmE 552707..553438 (+) 732 WP_413285220.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLKML_RS09995 (ACLKML_09995) gshB 553471..554424 (+) 954 WP_317718716.1 glutathione synthase -
  ACLKML_RS10000 (ACLKML_10000) - 554473..555036 (+) 564 WP_317718717.1 YqgE/AlgH family protein -
  ACLKML_RS10005 (ACLKML_10005) ruvX 555036..555467 (+) 432 WP_317718718.1 Holliday junction resolvase RuvX -
  ACLKML_RS10010 (ACLKML_10010) pilT 555518..556558 (-) 1041 WP_413285221.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKML_RS10015 (ACLKML_10015) - 556583..557293 (+) 711 WP_413285222.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKML_RS10020 (ACLKML_10020) proC 557318..558136 (+) 819 WP_413285223.1 pyrroline-5-carboxylate reductase -
  ACLKML_RS10025 (ACLKML_10025) - 558193..558747 (+) 555 WP_317718722.1 YggT family protein -
  ACLKML_RS10030 (ACLKML_10030) yggU 558747..559034 (+) 288 WP_413285224.1 DUF167 family protein YggU -
  ACLKML_RS10035 (ACLKML_10035) - 559070..559669 (+) 600 WP_317718724.1 XTP/dITP diphosphatase -
  ACLKML_RS10040 (ACLKML_10040) hemW 559673..560821 (+) 1149 WP_413285225.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37740.61 Da        Isoelectric Point: 7.2605

>NTDB_id=1090686 ACLKML_RS10010 WP_413285221.1 555518..556558(-) (pilT) [Vibrio sp. MA40-2]
MGITELLAFSVKHNASDLHLSAGVSPMIRIDGEVRKLDKPSLSDSEVHDVLLEVMSEAQQKEFAQKLEVDFCYDIASIGR
FRVNAFHQKRGCAIVFRSIPVDIPTLEALGCPSIFSQISIRDSGLILVTGPTGSGKSTTVAAMVNFINQHLNKHILTIED
PIEFVHSNIKCLINQREVHSDTHGFKVALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRV
IDVFPGNDKSMVRSMLSESLTTVIAQKLLKKKSGGRVACYEIMMATPAIRNLIRDNKVAQMYSIIQTGSASGMITMQQSA
NKLLKNGVVDAQEVSKKIGSDAIAFI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1090686 ACLKML_RS10010 WP_413285221.1 555518..556558(-) (pilT) [Vibrio sp. MA40-2]
ATGGGTATCACAGAGTTATTAGCTTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCAGCTGGCGTTTCTCCTAT
GATACGCATTGATGGAGAGGTTAGGAAGTTAGATAAGCCATCTTTGAGTGATTCTGAAGTACATGATGTATTACTTGAGG
TTATGAGCGAGGCACAACAGAAGGAATTTGCACAAAAACTAGAAGTGGATTTTTGTTATGATATTGCTTCAATTGGCCGA
TTTCGTGTCAATGCTTTTCATCAAAAGCGAGGATGCGCCATTGTATTTCGCAGCATTCCTGTCGATATTCCTACGTTGGA
GGCTTTAGGCTGTCCTAGTATTTTTAGTCAAATATCGATAAGGGATAGTGGGTTGATACTGGTGACAGGCCCGACCGGTT
CCGGTAAATCTACCACGGTTGCTGCTATGGTGAATTTTATAAACCAACACCTTAATAAGCATATTTTAACCATTGAAGAT
CCAATTGAATTTGTGCATTCAAATATTAAATGCCTTATCAATCAGCGTGAGGTTCATAGCGATACACATGGTTTTAAAGT
GGCTCTTCGTTCAGCGTTACGAGAGGATCCTGATGTCATTTTAGTAGGAGAGTTAAGAGATCAAGAAACTATAAGCTTGG
CATTAACCGCCGCAGAAACAGGGCATTTAGTTTTTGCGACCCTACATACTAGCTCTGCAGCCAAAACTATCGATAGAGTG
ATTGATGTGTTCCCGGGTAATGATAAGTCGATGGTGCGTAGTATGCTTTCTGAATCGTTAACGACAGTAATTGCTCAGAA
GTTACTAAAGAAAAAATCGGGTGGACGTGTCGCTTGTTATGAAATCATGATGGCGACACCTGCTATTCGAAATCTGATTC
GTGATAACAAAGTGGCGCAAATGTATTCGATTATTCAAACAGGTTCTGCATCAGGCATGATCACAATGCAACAAAGCGCC
AATAAGTTACTAAAGAATGGCGTTGTTGATGCACAAGAAGTCAGTAAAAAGATAGGGTCTGATGCGATAGCATTTATTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.072

99.711

0.749
  pilT Vibrio cholerae strain A1552

75.072

99.711

0.749
  pilT Acinetobacter nosocomialis M2

64.458

95.954

0.618
  pilT Acinetobacter baumannii D1279779

64.458

95.954

0.618
  pilT Acinetobacter baumannii strain A118

64.458

95.954

0.618
  pilT Acinetobacter baylyi ADP1

64.242

95.376

0.613
  pilT Pseudomonas stutzeri DSM 10701

61.721

97.399

0.601
  pilT Neisseria meningitidis 8013

59.483

100

0.598
  pilT Pseudomonas aeruginosa PAK

61.424

97.399

0.598
  pilT Legionella pneumophila strain Lp02

62.006

95.087

0.59
  pilT Legionella pneumophila strain ERS1305867

62.006

95.087

0.59
  pilT Neisseria gonorrhoeae MS11

58.501

100

0.587
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.889

91.04

0.445
  pilU Vibrio cholerae strain A1552

40.419

96.532

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.323

96.532

0.37

Multiple sequence alignment