Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACK2VK_RS05145 Genome accession   NZ_CP176650
Coordinates   1036374..1037354 (+) Length   326 a.a.
NCBI ID   WP_023247106.1    Uniprot ID   A0A8E9YEH3
Organism   Salmonella enterica subsp. enterica strain GTVSS-030     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1031374..1042354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2VK_RS05115 (ACK2VK_05115) ansB 1031888..1032934 (+) 1047 WP_023247102.1 L-asparaginase 2 -
  ACK2VK_RS05120 (ACK2VK_05120) hemW 1033050..1034186 (-) 1137 WP_053508151.1 radical SAM family heme chaperone HemW -
  ACK2VK_RS05125 (ACK2VK_05125) - 1034179..1034772 (-) 594 WP_079813140.1 XTP/dITP diphosphatase -
  ACK2VK_RS05130 (ACK2VK_05130) yggU 1034780..1035070 (-) 291 WP_023247104.1 DUF167 family protein YggU -
  ACK2VK_RS05135 (ACK2VK_05135) - 1035067..1035633 (-) 567 WP_001094848.1 YggT family protein -
  ACK2VK_RS05140 (ACK2VK_05140) - 1035652..1036356 (-) 705 WP_409528887.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACK2VK_RS05145 (ACK2VK_05145) pilT 1036374..1037354 (+) 981 WP_023247106.1 type IV pilus twitching motility protein PilT Machinery gene
  ACK2VK_RS05150 (ACK2VK_05150) - 1037486..1038172 (+) 687 WP_023247107.1 IclR family transcriptional regulator -
  ACK2VK_RS05155 (ACK2VK_05155) ruvX 1038220..1038636 (-) 417 WP_023247108.1 Holliday junction resolvase RuvX -
  ACK2VK_RS05160 (ACK2VK_05160) - 1038636..1039199 (-) 564 WP_053508147.1 YqgE/AlgH family protein -
  ACK2VK_RS05165 (ACK2VK_05165) gshB 1039415..1040362 (-) 948 WP_053528725.1 glutathione synthase -
  ACK2VK_RS05170 (ACK2VK_05170) rsmE 1040382..1041113 (-) 732 WP_023247111.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACK2VK_RS05175 (ACK2VK_05175) endA 1041189..1041896 (-) 708 WP_154714868.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36113.30 Da        Isoelectric Point: 7.5388

>NTDB_id=1081950 ACK2VK_RS05145 WP_023247106.1 1036374..1037354(+) (pilT) [Salmonella enterica subsp. enterica strain GTVSS-030]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLAGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGSGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYASKRCLIQQREINQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRGQLAGSLRAVLSQKLEPDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1081950 ACK2VK_RS05145 WP_023247106.1 1036374..1037354(+) (pilT) [Salmonella enterica subsp. enterica strain GTVSS-030]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGGATGGAGATCGCGCCTTTTACTGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTTTCGCTGGCCGGTACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTCTTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCAGCACTGCTTGCCAGTGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCAGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGGTATCTTAATCAACATGCCGATAAACATATTCTGACGCTGGAAGATCCTATTGAG
TACCGTTACGCCAGCAAGCGTTGCCTGATACAGCAGCGGGAGATCAACCAGCATTGCGCCACTTTCGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGTTGCGTGACAGCGAAACCATTCGCCTGGCGCTGACAG
CGGCGGAAACGGGACATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTGGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGTGGCCAACTGGCGGGCAGCCTGCGCGCCGTACTATCGCAAAAACTGGAGCC
TGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGAGAAGGTA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACGTTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

45.283

97.546

0.442
  pilT Legionella pneumophila strain ERS1305867

45.283

97.546

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.145

100

0.368

Multiple sequence alignment