Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJJVB_RS05380 Genome accession   NZ_CP174502
Coordinates   1155416..1155811 (+) Length   131 a.a.
NCBI ID   WP_407733172.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain ISHS.CFo     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1150416..1160811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJVB_RS05355 (ACJJVB_05355) yacG 1150507..1150716 (-) 210 WP_089390516.1 DNA gyrase inhibitor YacG -
  ACJJVB_RS05360 (ACJJVB_05360) coaE 1150713..1151321 (-) 609 WP_089390517.1 dephospho-CoA kinase -
  ACJJVB_RS05365 (ACJJVB_05365) pilD 1151390..1152262 (-) 873 WP_407733169.1 prepilin peptidase Machinery gene
  ACJJVB_RS05370 (ACJJVB_05370) pilC 1152265..1153482 (-) 1218 WP_407733170.1 type II secretion system F family protein Machinery gene
  ACJJVB_RS05375 (ACJJVB_05375) pilB 1153486..1155189 (-) 1704 WP_407733171.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJJVB_RS05380 (ACJJVB_05380) pilA 1155416..1155811 (+) 396 WP_407733172.1 pilin Machinery gene
  ACJJVB_RS05385 (ACJJVB_05385) - 1156026..1157768 (+) 1743 WP_407733173.1 tetratricopeptide repeat protein -
  ACJJVB_RS05390 (ACJJVB_05390) - 1157769..1158644 (+) 876 WP_407733174.1 glycosyltransferase family 2 protein -
  ACJJVB_RS05395 (ACJJVB_05395) - 1158646..1159734 (+) 1089 WP_407733175.1 UDP-N-acetylglucosamine 2-epimerase -
  ACJJVB_RS05400 (ACJJVB_05400) - 1159803..1160714 (-) 912 WP_407733176.1 YbhN family protein -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 13487.49 Da        Isoelectric Point: 5.0175

>NTDB_id=1077395 ACJJVB_RS05380 WP_407733172.1 1155416..1155811(+) (pilA) [Pseudomonas citronellolis strain ISHS.CFo]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYSKYQAKAKVTAGLAEISAGKTAFETKVNEGETISAVGDIGLAATTSNCNI
GVSGTTITCSLLNAPSQVSTGVITWTRNASTGEWACTVTNIDDEYAPKACQ

Nucleotide


Download         Length: 396 bp        

>NTDB_id=1077395 ACJJVB_RS05380 WP_407733172.1 1155416..1155811(+) (pilA) [Pseudomonas citronellolis strain ISHS.CFo]
ATGAAAGCTCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTGGTTGCGATCATTGGTATCTTGGCTGCTATCGCCAT
CCCGGCTTACAGCAAGTATCAGGCTAAGGCTAAAGTTACCGCTGGTTTGGCTGAGATTTCTGCAGGTAAGACTGCATTTG
AAACTAAAGTTAATGAAGGTGAAACTATCAGCGCTGTTGGGGATATTGGTCTCGCTGCAACCACGTCTAACTGCAATATC
GGTGTGAGTGGTACTACTATCACCTGCTCGCTGCTTAACGCGCCTTCCCAAGTAAGCACCGGTGTTATTACTTGGACTCG
TAACGCTTCTACTGGTGAGTGGGCGTGCACTGTTACCAATATTGATGATGAATATGCACCGAAGGCTTGTCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.201

100

0.511
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.143

100

0.504
  pilA Vibrio cholerae C6706

41.892

100

0.473
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.473
  pilA Vibrio cholerae strain A1552

41.892

100

0.473
  comP Acinetobacter baylyi ADP1

40.397

100

0.466
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.776

100

0.458
  pilA2 Legionella pneumophila str. Paris

42.537

100

0.435
  pilA Pseudomonas aeruginosa PAK

37.584

100

0.427
  pilA2 Legionella pneumophila strain ERS1305867

41.791

100

0.427
  pilA Vibrio parahaemolyticus RIMD 2210633

41.935

94.656

0.397
  pilA Haemophilus influenzae 86-028NP

42.5

91.603

0.389

Multiple sequence alignment