Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJJVB_RS05375 Genome accession   NZ_CP174502
Coordinates   1153486..1155189 (-) Length   567 a.a.
NCBI ID   WP_407733171.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain ISHS.CFo     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1148486..1160189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJVB_RS05340 (ACJJVB_05340) - 1149199..1149414 (+) 216 WP_089390514.1 hypothetical protein -
  ACJJVB_RS05345 (ACJJVB_05345) - 1149702..1149815 (-) 114 Protein_1050 PIN domain nuclease -
  ACJJVB_RS05350 (ACJJVB_05350) - 1149815..1150054 (-) 240 WP_275627642.1 ribbon-helix-helix domain-containing protein -
  ACJJVB_RS05355 (ACJJVB_05355) yacG 1150507..1150716 (-) 210 WP_089390516.1 DNA gyrase inhibitor YacG -
  ACJJVB_RS05360 (ACJJVB_05360) coaE 1150713..1151321 (-) 609 WP_089390517.1 dephospho-CoA kinase -
  ACJJVB_RS05365 (ACJJVB_05365) pilD 1151390..1152262 (-) 873 WP_407733169.1 prepilin peptidase Machinery gene
  ACJJVB_RS05370 (ACJJVB_05370) pilC 1152265..1153482 (-) 1218 WP_407733170.1 type II secretion system F family protein Machinery gene
  ACJJVB_RS05375 (ACJJVB_05375) pilB 1153486..1155189 (-) 1704 WP_407733171.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJJVB_RS05380 (ACJJVB_05380) pilA 1155416..1155811 (+) 396 WP_407733172.1 pilin Machinery gene
  ACJJVB_RS05385 (ACJJVB_05385) - 1156026..1157768 (+) 1743 WP_407733173.1 tetratricopeptide repeat protein -
  ACJJVB_RS05390 (ACJJVB_05390) - 1157769..1158644 (+) 876 WP_407733174.1 glycosyltransferase family 2 protein -
  ACJJVB_RS05395 (ACJJVB_05395) - 1158646..1159734 (+) 1089 WP_407733175.1 UDP-N-acetylglucosamine 2-epimerase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62540.66 Da        Isoelectric Point: 5.5976

>NTDB_id=1077394 ACJJVB_RS05375 WP_407733171.1 1153486..1155189(-) (pilB) [Pseudomonas citronellolis strain ISHS.CFo]
MNDQVVLSGLARQLVIAELLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGIAFCDLGAIDKDGLPKE
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKLFESATGGLDDLGDVDLE
GLDIESGDAERKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDIPPETLRHEGFP
EERIGSLKLYAPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1077394 ACJJVB_RS05375 WP_407733171.1 1153486..1155189(-) (pilB) [Pseudomonas citronellolis strain ISHS.CFo]
ATGAACGATCAAGTCGTGCTCTCCGGCCTCGCCCGTCAACTAGTGATCGCCGAGCTTCTTGACGAGAAGAGCGCCCAGCA
GGCGCAACAACAGGCGCAGCGTAATAAGCTGTCTTTGGTCACCTATCTTGTGCAGAACAAGCTGGTGAAAAGCCGTCCAT
TGACGGAACTGGCAGCTGACCAGTTCGGCATTGCTTTCTGCGACCTAGGCGCCATCGACAAGGACGGCCTTCCCAAGGAA
CTGATCAGCGAAAAACTGGTGCGCCAGCACCGCGTCATTCCGCTCTGGCGTCGTGGAAACAAACTCTTTGTGGGGATCTC
CGACCCCACCAATCACCAAGCCATCACCGATGTTCAGTTCAGTACAGGCTTGACCACCGAAGCTATTCTGGTTGAGGACG
ACAAACTCGGTGATGCAATCGAGAAACTCTTCGAGAGCGCCACCGGGGGGCTGGACGACCTGGGCGACGTCGACCTGGAA
GGCCTGGACATCGAAAGCGGCGACGCTGAGCGCAAGGAAGAGAGCATTGGCGGCGACGCAGATGACGCGCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCCATCAAGGGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAGAAAATCTACC
GGGTGCGCTTCCGTACCGACGGCATGCTGCATGAAGTGGCTAAACCACCGATTCAGTTGGCCAGCCGAATTTCCGCGCGT
CTCAAAGTAATGGCGGGCCTGGATATTTCCGAGCGGCGCAAGCCCCAGGACGGCCGTATCAAGATGCGAGTGTCCAAGAG
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCTACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTTGATTCGGCCA
GCGCACAGATGGGCATCGATGCCCTCGGCTATGAGGACGCACAGAAACAACTCTATCTGGAAGCTCTGAAACAACCGCAA
GGCATGATCCTAGTGACCGGCCCGACTGGGTCAGGCAAAACCGTATCCCTGTATACTGGTCTGAACATCCTCAACACCCC
GGACATCAATATCTCCACCGCAGAAGACCCGGTGGAAATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCCCGCC
AGGGCATGGACTTCGCCCAGGCCTTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTTGGCGAGATCCGCGAC
CTAGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACTCTGACACGCCTGCTGAACATGGGTGTAGCTTCATTCAACTTGGCCACGTCAACCAACCTGATCATCGCTCAAC
GCTTGGCACGCAAGCTTTGTCCACATTGTAAAAAAGAACACGACATCCCTCCCGAAACCCTGCGCCACGAAGGCTTCCCC
GAGGAAAGAATCGGCAGCCTCAAGCTTTACGCTCCTGTGGGGTGCGAAAATTGCAAGAACGGCTACAAAGGTCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCAGCGCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.447

99.471

0.571
  pilB Acinetobacter baumannii D1279779

57.345

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.55
  pilB Vibrio cholerae strain A1552

50.799

99.295

0.504
  pilB Vibrio campbellii strain DS40M4

50.265

99.647

0.501
  pilF Neisseria gonorrhoeae MS11

49.559

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.107

95.591

0.489
  pilF Thermus thermophilus HB27

39.474

100

0.397
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

93.298

0.377

Multiple sequence alignment