Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACJEH8_RS09540 Genome accession   NZ_CP174244
Coordinates   2116294..2116707 (-) Length   137 a.a.
NCBI ID   WP_011215877.1    Uniprot ID   Q5WVD6
Organism   Legionella pneumophila strain SPF580     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2111294..2121707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEH8_RS09525 (ACJEH8_09525) letS 2111612..2114344 (+) 2733 WP_011215874.1 two-component system sensor histidine kinase LetS Regulator
  ACJEH8_RS09530 (ACJEH8_09530) - 2114442..2115686 (+) 1245 WP_011215875.1 6-phosphofructokinase -
  ACJEH8_RS09535 (ACJEH8_09535) pilA2 2115683..2116096 (-) 414 WP_011215876.1 pilin Machinery gene
  ACJEH8_RS09540 (ACJEH8_09540) pilA2 2116294..2116707 (-) 414 WP_011215877.1 pilin Machinery gene
  ACJEH8_RS09545 (ACJEH8_09545) - 2117009..2117326 (+) 318 WP_011215878.1 BolA family transcriptional regulator -
  ACJEH8_RS09550 (ACJEH8_09550) - 2117401..2118822 (+) 1422 WP_011215879.1 APC family permease -
  ACJEH8_RS09555 (ACJEH8_09555) - 2118951..2120381 (-) 1431 WP_011215880.1 cellulase family glycosylhydrolase -
  ACJEH8_RS09560 (ACJEH8_09560) kdsB 2120629..2121381 (-) 753 WP_011215881.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  ACJEH8_RS09565 (ACJEH8_09565) - 2121378..2121557 (-) 180 WP_011215882.1 Trm112 family protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14041.20 Da        Isoelectric Point: 8.4715

>NTDB_id=1076478 ACJEH8_RS09540 WP_011215877.1 2116294..2116707(-) (pilA2) [Legionella pneumophila strain SPF580]
MRQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVSEGLQMASSAKLAVSETAITNNALPPTQAATGYVSPAATANV
SNIAIGANGVITITYTQAAGGGTIVMTPALDANNGDVTWNCTGGTLLAKYRPASCRP

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1076478 ACJEH8_RS09540 WP_011215877.1 2116294..2116707(-) (pilA2) [Legionella pneumophila strain SPF580]
ATGAGACAAAAGGGTTTTACATTAATTGAATTGATGATAGTCGTAGCGATTATTGGCATTTTGGCAGCAATCGCTATACC
AGCCTATCAAGATTACACAATACGGGCTCGTGTCAGCGAGGGGTTACAGATGGCAAGCTCAGCCAAGCTTGCTGTGTCTG
AAACGGCTATCACGAACAATGCGTTACCTCCTACTCAGGCAGCAACGGGCTACGTGTCTCCCGCTGCCACGGCTAATGTG
TCAAACATTGCGATAGGCGCTAACGGTGTGATAACCATCACGTATACCCAAGCAGCAGGTGGCGGAACGATTGTTATGAC
CCCTGCGCTGGATGCCAACAACGGTGATGTCACCTGGAATTGTACTGGCGGTACCTTATTGGCTAAATACCGACCTGCCA
GTTGCAGACCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WVD6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

90.511

100

0.905
  pilA2 Legionella pneumophila strain ERS1305867

89.781

100

0.898
  pilA Ralstonia pseudosolanacearum GMI1000

50.311

100

0.591
  comP Acinetobacter baylyi ADP1

52.055

100

0.555
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.766

100

0.518
  pilE Neisseria gonorrhoeae strain FA1090

37.58

100

0.431
  pilE Neisseria gonorrhoeae MS11

37.58

100

0.431
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.431
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.912

99.27

0.416
  pilA/pilA1 Eikenella corrodens VA1

36.601

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.568

100

0.401
  pilA Vibrio cholerae strain A1552

36.364

100

0.38
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.38
  pilA Vibrio cholerae C6706

36.364

100

0.38
  pilA Glaesserella parasuis strain SC1401

38.346

97.08

0.372

Multiple sequence alignment