Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJBUE_RS17700 Genome accession   NZ_CP174148
Coordinates   3721265..3722401 (-) Length   378 a.a.
NCBI ID   WP_078222022.1    Uniprot ID   -
Organism   Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3716265..3727401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJBUE_RS17675 (ACJBUE_17675) - 3717993..3718528 (-) 536 Protein_3480 pilus assembly protein -
  ACJBUE_RS17680 (ACJBUE_17680) - 3718532..3719554 (-) 1023 WP_078222018.1 PilW family protein -
  ACJBUE_RS17685 (ACJBUE_17685) pilV 3719551..3720141 (-) 591 WP_078222019.1 type IV pilus modification protein PilV -
  ACJBUE_RS17690 (ACJBUE_17690) - 3720138..3720626 (-) 489 WP_078222020.1 GspH/FimT family pseudopilin -
  ACJBUE_RS17695 (ACJBUE_17695) - 3720630..3721070 (-) 441 WP_078222021.1 type IV pilin protein -
  ACJBUE_RS17700 (ACJBUE_17700) pilU 3721265..3722401 (-) 1137 WP_078222022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJBUE_RS17705 (ACJBUE_17705) - 3722454..3723496 (-) 1043 Protein_3486 type IV pilus twitching motility protein PilT -
  ACJBUE_RS17710 (ACJBUE_17710) - 3723618..3724340 (+) 723 WP_075463463.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJBUE_RS17715 (ACJBUE_17715) proC 3724375..3725199 (+) 825 WP_013211543.1 pyrroline-5-carboxylate reductase -
  ACJBUE_RS17720 (ACJBUE_17720) - 3725212..3725490 (+) 279 WP_013211542.1 hypothetical protein -
  ACJBUE_RS17725 (ACJBUE_17725) ubiA 3725487..3726365 (-) 879 WP_078222023.1 4-hydroxybenzoate octaprenyltransferase -
  ACJBUE_RS17730 (ACJBUE_17730) - 3726438..3726938 (-) 501 WP_003271865.1 Dps family protein -
  ACJBUE_RS17735 (ACJBUE_17735) - 3727100..3727342 (-) 243 WP_013211540.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42090.59 Da        Isoelectric Point: 7.1068

>NTDB_id=1074549 ACJBUE_RS17700 WP_078222022.1 3721265..3722401(-) (pilU) [Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01]
MLDREAATKYIHELLQLMVNSRGSDLFITSEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREFEGSFECNFA
IVSGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAVMVGYRNAHSYG
HIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANSS
NQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVADLIFKGEVHELKEVMKKSREQG
MVSFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1074549 ACJBUE_RS17700 WP_078222022.1 3721265..3722401(-) (pilU) [Ralstonia syzygii subsp. celebesensis strain UGMSLYSS_Db01]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGATTTGTT
CATCACCTCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAGTTGCGCGAGTTCGAAGGCTCCTTCGAGTGCAACTTTGCC
ATCGTGTCCGGCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCATCAA
CACCAAGATCCCGACGCTCGGCGAACTGGATCTGCCGCCCATCCTCAACGAGGTCGCGATGAGCAAGCGCGGGCTCGTGG
TCGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGTGATGGTCGGCTACCGCAACGCGCATTCGTACGGC
CACATCATCACCATTGAAGACCCCGTGGAATACGTGCATGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGGCGT
GGATACGGAGTCGTGGCACGTGGCGCTGAAGAACACGTTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGTCACTTGTGCCTGGCCACGCTGCACGCCAACAGCTCG
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGTTGCTGATCGACCTGTCCCTGAACCT
GCGCGCCATGATCGCGCAGCGGCTGCTGCCGCGCAAAGGCAGGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGGGCA
CGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGGTGCATGAGCTCAAGGAGGTCATGAAGAAGTCCCGCGAGCAGGGG
ATGGTCTCGTTCGACCAGGCGCTGTTCGAACTCTATGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAATGCGGA
CTCGCTGAACGATCTGCGTCTGCAGATCAAGCTGCACAGCAAGCGCGGCGGGCCGGTGGACCTGACGGCAGGCACCGAGC
ATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.04

93.386

0.579
  pilU Vibrio cholerae strain A1552

55.807

93.386

0.521
  pilU Acinetobacter baylyi ADP1

54.596

94.974

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

42.433

89.153

0.378
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Acinetobacter baylyi ADP1

43.789

85.185

0.373
  pilT Vibrio cholerae strain A1552

44.479

83.862

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.479

83.862

0.373
  pilT Acinetobacter nosocomialis M2

42.947

84.392

0.362
  pilT Acinetobacter baumannii D1279779

42.947

84.392

0.362
  pilT Acinetobacter baumannii strain A118

42.947

84.392

0.362

Multiple sequence alignment