Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACI3L9_RS06120 Genome accession   NZ_CP173385
Coordinates   1376230..1377270 (-) Length   346 a.a.
NCBI ID   WP_005425810.1    Uniprot ID   A0A9X3MNX0
Organism   Vibrio campbellii strain LB198     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1371230..1382270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L9_RS06090 (ACI3L9_06090) endA 1371326..1372021 (+) 696 WP_005425828.1 deoxyribonuclease I -
  ACI3L9_RS06095 (ACI3L9_06095) rsmE 1372168..1372899 (+) 732 WP_005425825.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACI3L9_RS06100 (ACI3L9_06100) gshB 1372914..1373864 (+) 951 WP_079400969.1 glutathione synthase -
  ACI3L9_RS06105 (ACI3L9_06105) - 1373994..1374557 (+) 564 WP_010450344.1 YqgE/AlgH family protein -
  ACI3L9_RS06110 (ACI3L9_06110) ruvX 1374605..1375030 (+) 426 WP_005425820.1 Holliday junction resolvase RuvX -
  ACI3L9_RS06115 (ACI3L9_06115) pilU 1375092..1376198 (-) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACI3L9_RS06120 (ACI3L9_06120) pilT 1376230..1377270 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  ACI3L9_RS06125 (ACI3L9_06125) - 1377299..1378009 (+) 711 WP_077200191.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACI3L9_RS06130 (ACI3L9_06130) proC 1378140..1378958 (+) 819 WP_079400968.1 pyrroline-5-carboxylate reductase -
  ACI3L9_RS06135 (ACI3L9_06135) - 1379012..1379569 (+) 558 WP_005425806.1 YggT family protein -
  ACI3L9_RS06140 (ACI3L9_06140) yggU 1379569..1379859 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  ACI3L9_RS06145 (ACI3L9_06145) - 1379919..1380350 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  ACI3L9_RS06150 (ACI3L9_06150) - 1380456..1381058 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -
  ACI3L9_RS06155 (ACI3L9_06155) hemW 1381077..1382249 (+) 1173 WP_079400967.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38634.12 Da        Isoelectric Point: 6.5043

>NTDB_id=1071862 ACI3L9_RS06120 WP_005425810.1 1376230..1377270(-) (pilT) [Vibrio campbellii strain LB198]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1071862 ACI3L9_RS06120 WP_005425810.1 1376230..1377270(-) (pilT) [Vibrio campbellii strain LB198]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGACTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACTATCCCAAGTAGCATTCCAACATTGGA
AGAGTTAGAAGCTCCTGAGATCTTCAGAAAAATCGCCAATGCGGAAAAAGGTTTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACTACCTTGGCTGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGCAGCGCGCTACGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCGGCAGCGAAAACCATCGACCGTATT
ATTGACGTCTTCCCTGGTAGCGACAAAGACATGGTGCGTTCGATGCTGTCTGAATCATTGCGTTCGGTTATCGCGCAAAA
ACTTCTGAAACGTAACGGCGGCGGTCGAATCGCGTGTCACGAAATCATGATGGCTACGCCTGCGATCCGTAACCTTATTC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACTGGTGCGGCGCATGGTATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.118

98.266

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.503
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39

Multiple sequence alignment