Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACISH4_RS03680 Genome accession   NZ_CP173312
Coordinates   758720..759700 (+) Length   326 a.a.
NCBI ID   WP_001055657.1    Uniprot ID   A0A715N6Z8
Organism   Salmonella enterica subsp. enterica serovar Typhi strain 1CTRMumbai     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 753720..764700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACISH4_RS03650 (ACISH4_03655) - 754298..755305 (+) 1008 WP_000252198.1 DUF1202 domain-containing protein -
  ACISH4_RS03655 (ACISH4_03660) hemW 755396..756532 (-) 1137 WP_001096530.1 radical SAM family heme chaperone HemW -
  ACISH4_RS03660 (ACISH4_03665) - 756525..757118 (-) 594 WP_001174773.1 XTP/dITP diphosphatase -
  ACISH4_RS03665 (ACISH4_03670) yggU 757126..757416 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  ACISH4_RS03670 (ACISH4_03675) - 757413..757979 (-) 567 WP_001094848.1 YggT family protein -
  ACISH4_RS03675 (ACISH4_03680) - 757998..758702 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACISH4_RS03680 (ACISH4_03685) pilT 758720..759700 (+) 981 WP_001055657.1 type IV pilus twitching motility protein PilT Machinery gene
  ACISH4_RS03685 (ACISH4_03690) - 759830..760516 (+) 687 WP_000098333.1 global regulatory protein -
  ACISH4_RS03690 (ACISH4_03695) ruvX 760563..760979 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  ACISH4_RS03695 (ACISH4_03700) - 760979..761542 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  ACISH4_RS03700 (ACISH4_03705) gshB 761758..762705 (-) 948 WP_000593242.1 glutathione synthase -
  ACISH4_RS03705 (ACISH4_03710) rsmE 762725..763456 (-) 732 WP_001800534.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACISH4_RS03710 (ACISH4_03715) endA 763533..764240 (-) 708 WP_000286121.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36112.28 Da        Isoelectric Point: 7.5355

>NTDB_id=1071463 ACISH4_RS03680 WP_001055657.1 758720..759700(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhi strain 1CTRMumbai]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLAIRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1071463 ACISH4_RS03680 WP_001055657.1 758720..759700(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhi strain 1CTRMumbai]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGATACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A715N6Z8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368

Multiple sequence alignment