Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACG2K1_RS04995 Genome accession   NZ_CP173024
Coordinates   993533..994645 (+) Length   370 a.a.
NCBI ID   WP_198344885.1    Uniprot ID   -
Organism   Neisseria sp. 23W00296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 988533..999645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG2K1_RS04980 (ACG2K1_04980) cls 990422..991915 (+) 1494 WP_395428403.1 cardiolipin synthase -
  ACG2K1_RS04985 (ACG2K1_04985) - 992478..993062 (+) 585 WP_395428518.1 class I SAM-dependent methyltransferase -
  ACG2K1_RS04990 (ACG2K1_04990) - 993059..993430 (+) 372 WP_009426893.1 SirB2 family protein -
  ACG2K1_RS04995 (ACG2K1_04995) pilU 993533..994645 (+) 1113 WP_198344885.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG2K1_RS05000 (ACG2K1_05000) - 994742..995059 (+) 318 WP_009426895.1 hypothetical protein -
  ACG2K1_RS05005 (ACG2K1_05005) ccoS 995059..995268 (+) 210 WP_009426896.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  ACG2K1_RS05010 (ACG2K1_05010) - 995265..996560 (+) 1296 WP_395428404.1 MFS transporter -
  ACG2K1_RS05015 (ACG2K1_05015) - 996675..997025 (+) 351 WP_009426898.1 YkvA family protein -
  ACG2K1_RS05020 (ACG2K1_05020) - 997411..998349 (+) 939 WP_395428405.1 FecCD family ABC transporter permease -
  ACG2K1_RS05025 (ACG2K1_05025) - 998368..999096 (+) 729 WP_395428406.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41180.54 Da        Isoelectric Point: 6.0018

>NTDB_id=1069956 ACG2K1_RS04995 WP_198344885.1 993533..994645(+) (pilU) [Neisseria sp. 23W00296]
MGIKDTIFGWMRYMYEQKASDLFITAEFPPAVKLDGKLTPIADKPLTAGMCGAIAQALMTEKQREEFENTKECNFAVSLD
GVARFRVNAMIQRGAVALVIRIINSVIPKMDDLKLPEVLKKVAMQKRGLVIFVGGTGSGKSTSLAAMIDYRNENSYGHII
TIEDPIEYVHPHKNCIITQREVGVDTDNWFAALKNTLRQAPDVILIGEIRDQETMEYALAFAETGHLCMATLHANSSNQA
LDRIINFFPEEKRTQLLTDLSLNLQAFVSQRLVPKENGKGRVAAVEVLLQSPLISDLILRGEVHGIKEIMKKSTDIGMQT
FDQALYELFESGQISYAEAIKNADSANDLRLAIQLKSRRGQNAGIDFELL

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1069956 ACG2K1_RS04995 WP_198344885.1 993533..994645(+) (pilU) [Neisseria sp. 23W00296]
ATGGGCATCAAAGACACCATTTTCGGCTGGATGCGCTATATGTACGAACAAAAAGCGTCCGATCTCTTCATCACTGCCGA
ATTTCCGCCCGCCGTCAAACTCGACGGCAAACTCACGCCGATAGCCGACAAGCCGCTCACCGCCGGCATGTGCGGCGCCA
TCGCCCAGGCGCTGATGACCGAAAAGCAGCGCGAAGAATTTGAAAACACCAAAGAATGCAACTTTGCCGTCAGCCTCGAC
GGCGTGGCGCGTTTCCGCGTCAACGCCATGATCCAGCGCGGCGCCGTTGCCCTCGTTATCCGCATCATCAACAGCGTCAT
TCCGAAAATGGACGACCTCAAACTGCCCGAAGTGCTGAAAAAAGTGGCGATGCAGAAACGCGGGCTGGTGATTTTCGTCG
GCGGCACAGGCTCGGGCAAATCCACCTCGCTGGCGGCGATGATCGACTACCGCAACGAAAACAGCTACGGCCACATCATC
ACCATCGAAGACCCCATCGAATACGTCCACCCGCACAAAAACTGCATCATCACCCAGCGCGAAGTGGGCGTGGACACCGA
CAACTGGTTTGCCGCCCTGAAAAACACCCTGCGCCAGGCGCCCGACGTGATCCTCATCGGCGAGATCCGCGACCAGGAAA
CCATGGAATACGCGCTGGCCTTCGCCGAAACCGGCCACCTCTGCATGGCCACGCTGCACGCCAACAGTTCCAACCAGGCA
CTCGACCGCATCATCAACTTCTTCCCCGAAGAAAAACGCACCCAGCTTCTGACCGACCTGTCGCTCAATTTGCAGGCCTT
CGTCTCCCAGCGCCTCGTGCCCAAGGAAAACGGCAAAGGCCGTGTGGCGGCGGTGGAAGTGCTGCTGCAATCGCCGCTGA
TTTCCGACCTTATCCTGCGCGGCGAAGTGCACGGCATCAAGGAAATCATGAAAAAATCCACCGACATCGGCATGCAGACC
TTTGACCAGGCGCTGTACGAACTCTTCGAAAGCGGCCAGATTTCCTACGCCGAAGCGATTAAAAACGCCGATTCCGCCAA
CGACCTGCGTCTGGCCATCCAGCTCAAAAGCCGCAGGGGGCAGAACGCCGGCATCGACTTCGAGCTGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.383

93.784

0.576
  pilU Vibrio cholerae strain A1552

54.496

99.189

0.541
  pilU Acinetobacter baylyi ADP1

57.349

93.784

0.538
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.732

92.703

0.405
  pilT Pseudomonas aeruginosa PAK

43.114

90.27

0.389
  pilT Legionella pneumophila strain Lp02

44.172

88.108

0.389
  pilT Legionella pneumophila strain ERS1305867

44.172

88.108

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.515

90.27

0.384
  pilT Acinetobacter baumannii D1279779

42.945

88.108

0.378
  pilT Acinetobacter baumannii strain A118

42.945

88.108

0.378
  pilT Acinetobacter nosocomialis M2

42.945

88.108

0.378
  pilT Acinetobacter baylyi ADP1

41.071

90.811

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.337

88.919

0.368
  pilT Vibrio cholerae strain A1552

41.337

88.919

0.368
  pilT Neisseria meningitidis 8013

39.244

92.973

0.365

Multiple sequence alignment