Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RSP597_RS14320 Genome accession   NZ_CP172969
Coordinates   3001142..3002281 (+) Length   379 a.a.
NCBI ID   WP_003271877.1    Uniprot ID   A0AAP7ZNZ2
Organism   Ralstonia solanacearum strain P597     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2996142..3007281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS14290 (RSP597_014290) ubiA 2996371..2997246 (+) 876 WP_064045017.1 4-hydroxybenzoate octaprenyltransferase -
  RSP597_RS14300 (RSP597_014300) - 2998058..2998336 (-) 279 WP_064045018.1 hypothetical protein -
  RSP597_RS14305 (RSP597_014305) proC 2998345..2999169 (-) 825 WP_013205197.1 pyrroline-5-carboxylate reductase -
  RSP597_RS14310 (RSP597_014310) - 2999205..2999927 (-) 723 WP_097908838.1 YggS family pyridoxal phosphate-dependent enzyme -
  RSP597_RS14315 (RSP597_014315) pilT 3000049..3001092 (+) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene
  RSP597_RS14320 (RSP597_014320) pilU 3001142..3002281 (+) 1140 WP_003271877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RSP597_RS14325 (RSP597_014325) - 3002477..3002917 (+) 441 WP_043908211.1 type IV pilin protein -
  RSP597_RS14330 (RSP597_014330) - 3002921..3003409 (+) 489 WP_013205201.1 GspH/FimT family pseudopilin -
  RSP597_RS14335 (RSP597_014335) pilV 3003406..3003996 (+) 591 WP_064045019.1 type IV pilus modification protein PilV -
  RSP597_RS14340 (RSP597_014340) - 3003993..3005027 (+) 1035 WP_064045020.1 PilW family protein -
  RSP597_RS14345 (RSP597_014345) - 3005031..3005567 (+) 537 WP_064045021.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42235.66 Da        Isoelectric Point: 6.9314

>NTDB_id=1069777 RSP597_RS14320 WP_003271877.1 3001142..3002281(+) (pilU) [Ralstonia solanacearum strain P597]
MLDRDAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREYEDSFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTINTKIPTLTELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIVNFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVTFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHGKRGGPADLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1069777 RSP597_RS14320 WP_003271877.1 3001142..3002281(+) (pilU) [Ralstonia solanacearum strain P597]
ATGCTGGACCGCGACGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACCTGTT
CATCACCGCTGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAACTGCGCGAGTACGAAGACTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACCAAGATCCCGACGCTCACCGAACTGGACCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTCGTGGTGGGGGCGACGGGTTCGGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATACGTGCACGCACACCACAACTGCGTTGTGACGCAGCGCGAGGTGGG
CGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCGTCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCGATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAGGAAGGGCCGTGCGCCGGCGATCGAAATCCTGCTGG
GCACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAGTCTCGCGAGCAG
GGGATGGTCACGTTCGACCAGGCACTGTTCGAGCTCTATGAGGCGGACAAGATCACCTATGAAGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTCTGCAGATCAAGCTGCACGGCAAGCGCGGCGGGCCGGCGGATCTGACGGCCGGCACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.756

93.14

0.575
  pilU Acinetobacter baylyi ADP1

54.875

94.723

0.52
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.544

87.863

0.383
  pilT Legionella pneumophila strain ERS1305867

43.544

87.863

0.383
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Vibrio cholerae strain A1552

45.741

83.641

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.741

83.641

0.383
  pilT Acinetobacter baylyi ADP1

44.72

84.96

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372
  pilT Acinetobacter baumannii D1279779

43.887

84.169

0.369
  pilT Acinetobacter baumannii strain A118

43.887

84.169

0.369
  pilT Acinetobacter nosocomialis M2

43.887

84.169

0.369

Multiple sequence alignment