Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHMW6_RS04165 Genome accession   NZ_CP171977
Coordinates   761576..762757 (+) Length   393 a.a.
NCBI ID   WP_395405806.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 756576..767757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMW6_RS04135 (ACHMW6_04125) pilT 756587..757630 (-) 1044 WP_395405796.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMW6_RS04140 (ACHMW6_04130) - 757667..758375 (+) 709 Protein_724 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMW6_RS04145 (ACHMW6_04135) proC 758382..759218 (+) 837 WP_395405798.1 pyrroline-5-carboxylate reductase -
  ACHMW6_RS04150 (ACHMW6_04140) - 759215..759598 (-) 384 WP_395405800.1 YqjD family protein -
  ACHMW6_RS04155 (ACHMW6_04145) ubiA 759629..760504 (-) 876 WP_395405802.1 4-hydroxybenzoate octaprenyltransferase -
  ACHMW6_RS04160 (ACHMW6_04150) - 760539..761477 (-) 939 WP_395405804.1 hydrogen peroxide-inducible genes activator -
  ACHMW6_RS04165 (ACHMW6_04155) pilU 761576..762757 (+) 1182 WP_395405806.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMW6_RS04170 (ACHMW6_04160) - 762761..763240 (+) 480 WP_395405808.1 adenylyltransferase/cytidyltransferase family protein -
  ACHMW6_RS04175 (ACHMW6_04165) - 763253..763534 (+) 282 WP_395405810.1 hypothetical protein -
  ACHMW6_RS04180 (ACHMW6_04170) - 763752..763937 (+) 186 WP_395405812.1 hypothetical protein -
  ACHMW6_RS04185 (ACHMW6_04175) - 764061..764933 (-) 873 WP_395405814.1 REP-associated tyrosine transposase -
  ACHMW6_RS04190 (ACHMW6_04180) trmL 765038..765508 (-) 471 WP_395405816.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  ACHMW6_RS04195 (ACHMW6_04185) - 765510..766268 (-) 759 WP_395405818.1 phosphoribosyltransferase family protein -
  ACHMW6_RS04200 (ACHMW6_04190) - 766295..767217 (+) 923 Protein_736 methyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 393 a.a.        Molecular weight: 43818.91 Da        Isoelectric Point: 6.1525

>NTDB_id=1064691 ACHMW6_RS04165 WP_395405806.1 761576..762757(+) (pilU) [Pseudoduganella sp. UC29_106]
MSSTLGHEEAQSYIHKLLKAMHQVGGSDLFISADFPPSIKSQGSMRPLSQQRLTADVTRALAYAIMNDKQRREFETELEC
NFAISLPDVCRFRVNVFVQQQAVGMVIRTIASEIPNFEKLDLPEVLKEVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NSAGHIITVEDPVEYVHKNKGCLITHREVGVDTLSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTISEMIFKGNFHAIKEIMHKS
RELGMCTFDQALYELYNKGYIAYEEALRNADSANQLRLQIKLSGTRKSPDDGGGGGHGPGELSMQVDEEEEEE

Nucleotide


Download         Length: 1182 bp        

>NTDB_id=1064691 ACHMW6_RS04165 WP_395405806.1 761576..762757(+) (pilU) [Pseudoduganella sp. UC29_106]
ATGTCATCCACACTTGGGCACGAAGAAGCCCAGTCCTATATCCACAAGCTGCTGAAGGCCATGCACCAGGTCGGCGGCTC
CGACCTGTTCATTTCAGCCGATTTCCCGCCCAGTATCAAGTCGCAAGGCTCCATGCGGCCGCTCAGCCAGCAGCGCCTGA
CGGCGGATGTGACCCGCGCGCTGGCCTACGCCATCATGAACGACAAGCAGCGGCGCGAGTTCGAAACCGAACTCGAATGC
AACTTCGCCATCTCGCTGCCGGACGTGTGCCGTTTCCGCGTCAACGTCTTTGTGCAGCAGCAGGCGGTGGGCATGGTGAT
CCGTACCATCGCCTCCGAAATCCCGAATTTCGAGAAGCTGGACTTGCCGGAAGTGCTGAAGGAAGTGGTGATGACCAAGC
GCGGGTTGGTGCTGGTGGTGGGCGGCACGGGTTCCGGCAAATCGACCACGCTGGCGGCGATGATCGATTATCGAAATGCC
AACTCGGCGGGCCACATCATCACCGTGGAAGACCCGGTGGAATACGTCCACAAGAACAAGGGCTGCCTGATCACGCACCG
CGAAGTGGGCGTGGACACCCTAAGCTGGCACCATGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAAATCCGCGATACGGAAACCATGGAGCACGCGATCGCGTTTGCCGAAACGGGCCACCTGTGCCTGGGCACCCTGCAC
GCGAACAACGCCAACCAGACCATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCAACCAGCTGCTGATGGATTT
GTCGTCCAACCTGCGCGCCATCGTGTCGCAGCGCCTGATCCGCACCGAAGACGGCAAGGGTCGCAAGGCGGCGATCGAGA
TCCTGCTGAATACCCCGACCATTTCCGAAATGATCTTCAAGGGGAACTTCCACGCCATCAAGGAAATCATGCACAAGTCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCGCTGTACGAACTCTATAATAAAGGCTATATCGCCTATGAAGAGGCGCT
GCGCAATGCCGACTCGGCCAACCAGTTGCGCCTGCAGATCAAGCTGAGCGGCACGCGCAAGAGCCCCGATGACGGTGGCG
GCGGCGGGCACGGTCCCGGCGAATTGAGCATGCAGGTGGATGAAGAAGAGGAAGAAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.231

88.295

0.532
  pilU Acinetobacter baylyi ADP1

54.902

90.84

0.499
  pilU Vibrio cholerae strain A1552

51.801

91.858

0.476
  pilT Pseudomonas aeruginosa PAK

45.697

85.751

0.392
  pilT Acinetobacter baylyi ADP1

46.037

83.461

0.384
  pilT Legionella pneumophila strain ERS1305867

46.319

82.952

0.384
  pilT Legionella pneumophila strain Lp02

46.319

82.952

0.384
  pilT Pseudomonas stutzeri DSM 10701

44.214

85.751

0.379
  pilT Acinetobacter baumannii D1279779

44.745

84.733

0.379
  pilT Acinetobacter nosocomialis M2

44.745

84.733

0.379
  pilT Acinetobacter baumannii strain A118

44.745

84.733

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

85.751

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.277

84.478

0.374
  pilT Vibrio cholerae strain A1552

44.277

84.478

0.374

Multiple sequence alignment