Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFBML_RS13305 Genome accession   NZ_CP170491
Coordinates   2937082..2938122 (-) Length   346 a.a.
NCBI ID   WP_005425810.1    Uniprot ID   A0A9X3MNX0
Organism   Vibrio harveyi strain LcV6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2932082..2943122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFBML_RS13275 (ACFBML_13275) endA 2932178..2932873 (+) 696 WP_005449743.1 deoxyribonuclease I -
  ACFBML_RS13280 (ACFBML_13280) rsmE 2933020..2933751 (+) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFBML_RS13285 (ACFBML_13285) gshB 2933766..2934716 (+) 951 WP_005449741.1 glutathione synthase -
  ACFBML_RS13290 (ACFBML_13290) - 2934846..2935409 (+) 564 WP_005449740.1 YqgE/AlgH family protein -
  ACFBML_RS13295 (ACFBML_13295) ruvX 2935459..2935884 (+) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  ACFBML_RS13300 (ACFBML_13300) pilU 2935944..2937050 (-) 1107 WP_005449736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFBML_RS13305 (ACFBML_13305) pilT 2937082..2938122 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFBML_RS13310 (ACFBML_13310) - 2938151..2938861 (+) 711 WP_009697582.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFBML_RS13315 (ACFBML_13315) proC 2938993..2939811 (+) 819 WP_390338085.1 pyrroline-5-carboxylate reductase -
  ACFBML_RS13320 (ACFBML_13320) - 2939865..2940422 (+) 558 WP_005425806.1 YggT family protein -
  ACFBML_RS13325 (ACFBML_13325) yggU 2940422..2940712 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  ACFBML_RS13330 (ACFBML_13330) - 2940888..2941139 (+) 252 WP_005449728.1 hypothetical protein -
  ACFBML_RS13335 (ACFBML_13335) - 2941188..2941514 (-) 327 WP_005449725.1 hypothetical protein -
  ACFBML_RS13340 (ACFBML_13340) - 2941585..2941740 (-) 156 WP_005449723.1 hypothetical protein -
  ACFBML_RS13345 (ACFBML_13345) - 2941733..2941933 (-) 201 WP_005449722.1 hypothetical protein -
  ACFBML_RS13350 (ACFBML_13350) - 2942031..2942513 (-) 483 WP_017190983.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38634.12 Da        Isoelectric Point: 6.5043

>NTDB_id=1057696 ACFBML_RS13305 WP_005425810.1 2937082..2938122(-) (pilT) [Vibrio harveyi strain LcV6]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1057696 ACFBML_RS13305 WP_005425810.1 2937082..2938122(-) (pilT) [Vibrio harveyi strain LcV6]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCCCCAGAGATCTTCAGAAAGATTGCCAATGCTGAAAAAGGCCTGGTACTGGTTACTGGTCCAACGGGTT
CTGGTAAATCGACTACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCACTGCGCAGCGCATTACGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCAGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCGGAGTCGCTGCGTTCGGTTATTGCACAAAA
ACTGTTAAAACGTAATGGTGGTGGTCGTATTGCTTGTCATGAAATTATGATGGCGACACCTGCGATCCGTAACCTTATTC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACCGGTGCGGCGCATGGCATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCGCAAGGCATGGTATCGCGTGAAGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.377

99.711

0.861
  pilT Vibrio cholerae strain A1552

86.377

99.711

0.861
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

68.824

98.266

0.676
  pilT Pseudomonas stutzeri DSM 10701

69.027

97.977

0.676
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

64.118

98.266

0.63
  pilT Neisseria gonorrhoeae MS11

63.824

98.266

0.627
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.857

100

0.503
  pilU Vibrio cholerae strain A1552

41.617

96.532

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39

Multiple sequence alignment