Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFDAU_RS13910 Genome accession   NZ_CP170197
Coordinates   2801894..2803021 (+) Length   375 a.a.
NCBI ID   WP_413437495.1    Uniprot ID   -
Organism   Sulfuriferula sp. GW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2796894..2808021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFDAU_RS13885 (ACFDAU_13885) - 2798343..2798642 (-) 300 WP_413437490.1 DUF167 domain-containing protein -
  ACFDAU_RS13890 (ACFDAU_13890) - 2798655..2799230 (-) 576 WP_413437491.1 YggT family protein -
  ACFDAU_RS13895 (ACFDAU_13895) proC 2799293..2800099 (-) 807 WP_413437492.1 pyrroline-5-carboxylate reductase -
  ACFDAU_RS13900 (ACFDAU_13900) - 2800116..2800799 (-) 684 WP_413437493.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFDAU_RS13905 (ACFDAU_13905) pilT 2800833..2801876 (+) 1044 WP_413437494.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFDAU_RS13910 (ACFDAU_13910) pilU 2801894..2803021 (+) 1128 WP_413437495.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFDAU_RS13915 (ACFDAU_13915) - 2803311..2806058 (+) 2748 WP_413437496.1 EAL domain-containing protein -
  ACFDAU_RS13920 (ACFDAU_13920) - 2806065..2807216 (-) 1152 WP_413437497.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  ACFDAU_RS13925 (ACFDAU_13925) - 2807222..2807629 (-) 408 WP_413437498.1 CopD family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42052.34 Da        Isoelectric Point: 6.5812

>NTDB_id=1055511 ACFDAU_RS13910 WP_413437495.1 2801894..2803021(+) (pilU) [Sulfuriferula sp. GW1]
MDALKALHDLLRLMLSKKASDLFITAGFPPAIKVDGKIAPVSNQTLTPAHTREFARALMNDRQWADFEASHESNFAISPP
EIGRFRVNAFVQQARVGLVLRTITTRIPKFEELNLPPVLKDVAMVKRGLVIFVGGTGSGKSTSLAAMVGYRNENAQDHII
TIEDPVEYVHEHKKSIITQREVGVDTDNWFAALKNTLRQAPDVILIGEVRDRETMEYAIAFAETGHLCLTTLHANSANQA
LDRIINFFPEDRRQQLLMDLSLNLKSFISQRLIARKGAPGRVAAVEILLNSPLISELIFKGDVHEIKEIMSKSRELGMQT
FDHSLFDLYEAGMISYEDALRNADSLNDLRLKIKLQGQESRERDIMSGLDHLDIV

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1055511 ACFDAU_RS13910 WP_413437495.1 2801894..2803021(+) (pilU) [Sulfuriferula sp. GW1]
ATGGACGCCCTCAAAGCCTTGCACGATCTGCTGCGCCTGATGCTCAGCAAAAAAGCCTCGGATTTATTCATCACCGCGGG
CTTTCCTCCCGCCATCAAGGTGGACGGCAAGATAGCGCCCGTATCCAACCAGACCCTTACCCCCGCGCATACACGCGAGT
TTGCGCGGGCATTGATGAACGATCGCCAGTGGGCGGATTTTGAAGCAAGCCATGAAAGCAACTTTGCCATTTCCCCGCCG
GAAATCGGCCGTTTTCGTGTCAACGCATTTGTCCAGCAGGCACGCGTTGGCCTGGTATTGCGGACCATCACCACCAGGAT
TCCCAAGTTTGAGGAACTCAACCTGCCCCCGGTACTCAAGGATGTGGCCATGGTCAAACGCGGCCTGGTGATTTTTGTCG
GCGGTACCGGTTCGGGTAAATCCACTTCGCTCGCGGCGATGGTCGGCTACCGCAATGAAAATGCCCAGGATCACATCATT
ACCATCGAAGACCCGGTGGAGTATGTTCACGAACACAAGAAATCCATCATCACCCAGCGCGAAGTCGGCGTGGATACCGA
CAACTGGTTTGCGGCATTGAAAAACACCCTGCGTCAGGCCCCCGACGTGATCCTGATCGGCGAGGTGCGCGACCGGGAAA
CCATGGAATATGCCATCGCTTTTGCTGAAACCGGTCACCTGTGCCTGACCACGCTGCACGCCAACAGCGCCAATCAGGCG
CTGGACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCCAGCAATTGCTGATGGATTTGTCGCTGAATCTCAAGTCGTT
TATCTCGCAGCGCCTGATCGCCAGAAAAGGCGCCCCGGGACGCGTTGCAGCGGTCGAGATTTTGCTCAACTCGCCGTTGA
TTTCGGAACTGATATTTAAAGGCGATGTTCACGAGATCAAGGAGATCATGTCGAAATCACGCGAGCTGGGCATGCAGACT
TTCGACCACTCGCTGTTCGACCTCTACGAGGCGGGCATGATCAGCTACGAGGATGCGTTGCGCAATGCCGACTCCCTCAA
TGACCTCAGGCTGAAGATCAAGCTGCAAGGCCAGGAATCCAGAGAGCGCGATATCATGTCGGGACTGGATCACCTCGATA
TCGTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.04

94.133

0.584
  pilU Acinetobacter baylyi ADP1

56.825

95.733

0.544
  pilU Vibrio cholerae strain A1552

55.172

92.8

0.512
  pilT Pseudomonas aeruginosa PAK

44.179

89.333

0.395
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.867

0.392
  pilT Acinetobacter baumannii strain A118

42.9

88.267

0.379
  pilT Acinetobacter nosocomialis M2

42.9

88.267

0.379
  pilT Acinetobacter baumannii D1279779

42.9

88.267

0.379
  pilT Acinetobacter baylyi ADP1

42.598

88.267

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.791

89.333

0.373
  pilT Legionella pneumophila strain Lp02

41.39

88.267

0.365
  pilT Legionella pneumophila strain ERS1305867

41.39

88.267

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

88

0.365
  pilT Vibrio cholerae strain A1552

41.515

88

0.365

Multiple sequence alignment