Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEY3T_RS04235 Genome accession   NZ_CP169352
Coordinates   870505..871485 (+) Length   326 a.a.
NCBI ID   WP_020899201.1    Uniprot ID   V7IVS7
Organism   Salmonella enterica strain SA40     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 865505..876485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY3T_RS04205 (ACEY3T_04205) - 866082..867089 (+) 1008 WP_023138752.1 DUF1202 domain-containing protein -
  ACEY3T_RS04210 (ACEY3T_04210) hemW 867181..868317 (-) 1137 WP_023138753.1 radical SAM family heme chaperone HemW -
  ACEY3T_RS04215 (ACEY3T_04215) - 868310..868903 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACEY3T_RS04220 (ACEY3T_04220) yggU 868911..869201 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  ACEY3T_RS04225 (ACEY3T_04225) - 869198..869764 (-) 567 WP_001094848.1 YggT family protein -
  ACEY3T_RS04230 (ACEY3T_04230) - 869783..870487 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEY3T_RS04235 (ACEY3T_04235) pilT 870505..871485 (+) 981 WP_020899201.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEY3T_RS04240 (ACEY3T_04240) - 871615..872301 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  ACEY3T_RS04245 (ACEY3T_04245) ruvX 872348..872764 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  ACEY3T_RS04250 (ACEY3T_04250) - 872764..873327 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  ACEY3T_RS04255 (ACEY3T_04255) gshB 873543..874490 (-) 948 WP_000593248.1 glutathione synthase -
  ACEY3T_RS04260 (ACEY3T_04260) rsmE 874510..875241 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEY3T_RS04265 (ACEY3T_04265) endA 875318..876025 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36140.34 Da        Isoelectric Point: 7.5355

>NTDB_id=1050789 ACEY3T_RS04235 WP_020899201.1 870505..871485(+) (pilT) [Salmonella enterica strain SA40]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLSLTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1050789 ACEY3T_RS04235 WP_020899201.1 870505..871485(+) (pilT) [Salmonella enterica strain SA40]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAGCAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCTGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGTCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V7IVS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Pseudomonas stutzeri DSM 10701

36.036

100

0.368

Multiple sequence alignment