Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACEY30_RS03830 Genome accession   NZ_CP169336
Coordinates   787932..788912 (+) Length   326 a.a.
NCBI ID   WP_001055639.1    Uniprot ID   A0A8E6VVQ5
Organism   Salmonella enterica strain SA89     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782932..793912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY30_RS03800 (ACEY30_03800) - 783510..784517 (+) 1008 WP_000252199.1 DUF1202 domain-containing protein -
  ACEY30_RS03805 (ACEY30_03805) hemW 784608..785744 (-) 1137 WP_001096523.1 radical SAM family heme chaperone HemW -
  ACEY30_RS03810 (ACEY30_03810) - 785737..786330 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  ACEY30_RS03815 (ACEY30_03815) yggU 786338..786628 (-) 291 WP_001277204.1 DUF167 family protein YggU -
  ACEY30_RS03820 (ACEY30_03820) - 786625..787191 (-) 567 WP_001094842.1 YggT family protein -
  ACEY30_RS03825 (ACEY30_03825) - 787210..787914 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACEY30_RS03830 (ACEY30_03830) pilT 787932..788912 (+) 981 WP_001055639.1 type IV pilus twitching motility protein PilT Machinery gene
  ACEY30_RS03835 (ACEY30_03835) - 789045..789731 (+) 687 WP_000098338.1 IclR family transcriptional regulator -
  ACEY30_RS03840 (ACEY30_03840) ruvX 789778..790194 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  ACEY30_RS03845 (ACEY30_03845) - 790194..790757 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  ACEY30_RS03850 (ACEY30_03850) gshB 790973..791920 (-) 948 WP_000593248.1 glutathione synthase -
  ACEY30_RS03855 (ACEY30_03855) rsmE 791940..792671 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACEY30_RS03860 (ACEY30_03860) endA 792748..793455 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36150.37 Da        Isoelectric Point: 7.5116

>NTDB_id=1050661 ACEY30_RS03830 WP_001055639.1 787932..788912(+) (pilT) [Salmonella enterica strain SA89]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1050661 ACEY30_RS03830 WP_001055639.1 787932..788912(+) (pilT) [Salmonella enterica strain SA89]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGTCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.637

100

0.374

Multiple sequence alignment