Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABQ179_RS20095 Genome accession   NZ_CP167817
Coordinates   4654502..4655635 (+) Length   377 a.a.
NCBI ID   WP_104615100.1    Uniprot ID   A0A2S7C6U0
Organism   Xanthomonas dyei strain WHRI 5755     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4649502..4660635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ179_RS20070 (ABQ179_020070) - 4649668..4650861 (-) 1194 WP_349811757.1 cystathionine gamma-synthase -
  ABQ179_RS20075 (ABQ179_020075) - 4650951..4652321 (-) 1371 WP_349811758.1 pyridoxal-phosphate dependent enzyme -
  ABQ179_RS20080 (ABQ179_020080) - 4652560..4652778 (-) 219 WP_005991804.1 YdcH family protein -
  ABQ179_RS20085 (ABQ179_020085) - 4653132..4654022 (-) 891 WP_104615102.1 hypothetical protein -
  ABQ179_RS20090 (ABQ179_020090) - 4654019..4654402 (-) 384 WP_104615101.1 DUF4398 domain-containing protein -
  ABQ179_RS20095 (ABQ179_020095) pilU 4654502..4655635 (+) 1134 WP_104615100.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQ179_RS20100 (ABQ179_020100) maiA 4655917..4656579 (-) 663 WP_104615099.1 maleylacetoacetate isomerase -
  ABQ179_RS20105 (ABQ179_020105) - 4656623..4657681 (-) 1059 WP_211287656.1 fumarylacetoacetate hydrolase family protein -
  ABQ179_RS20110 (ABQ179_020110) - 4657988..4659373 (+) 1386 WP_055848080.1 DEAD/DEAH box helicase -
  ABQ179_RS20115 (ABQ179_020115) queD 4659525..4659878 (+) 354 WP_055850251.1 6-carboxytetrahydropterin synthase QueD -
  ABQ179_RS20120 (ABQ179_020120) - 4660070..4660405 (+) 336 WP_104615097.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41728.03 Da        Isoelectric Point: 6.1401

>NTDB_id=1039559 ABQ179_RS20095 WP_104615100.1 4654502..4655635(+) (pilU) [Xanthomonas dyei strain WHRI 5755]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1039559 ABQ179_RS20095 WP_104615100.1 4654502..4655635(+) (pilU) [Xanthomonas dyei strain WHRI 5755]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTTGGCAACACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGTCCGATGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
TGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGTTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCGGTGGTCTCGCAGCG
CCTGGTCAAGGGCGTGGATGGACGCCGCCGCCCCGCCACCGAGGTGTTGCTCAATACCCCGATGATCCGCGACCTGCTGC
GTCGTGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAGTCGTTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGACCGGTGAGATCGAGCAGGAAGAAGCGCTGCGTGCAGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGGCTGTCCGAAGGCGGCTCCGGCGAGCACGACCCATACGCGGATTACGAGTCGGCGTCGGCGCCGAAGATCAGTC
ACGGGTTTATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7C6U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361

Multiple sequence alignment