Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB9X52_RS18370 Genome accession   NZ_CP166433
Coordinates   3852013..3852993 (-) Length   326 a.a.
NCBI ID   WP_375601894.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 2024CK-00699     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3847013..3857993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9X52_RS18340 (AB9X52_18335) - 3847890..3848387 (+) 498 WP_375601890.1 SprT family zinc-dependent metalloprotease -
  AB9X52_RS18345 (AB9X52_18340) endA 3848482..3849189 (+) 708 WP_375601891.1 deoxyribonuclease I -
  AB9X52_RS18350 (AB9X52_18345) rsmE 3849241..3849972 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB9X52_RS18355 (AB9X52_18350) gshB 3850004..3850951 (+) 948 WP_375601892.1 glutathione synthase -
  AB9X52_RS18360 (AB9X52_18355) - 3851026..3851586 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  AB9X52_RS18365 (AB9X52_18360) ruvX 3851586..3852002 (+) 417 WP_375601893.1 Holliday junction resolvase RuvX -
  AB9X52_RS18370 (AB9X52_18365) pilT 3852013..3852993 (-) 981 WP_375601894.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9X52_RS18375 (AB9X52_18370) - 3853011..3853712 (+) 702 WP_047050755.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9X52_RS18380 (AB9X52_18375) - 3853734..3854300 (+) 567 WP_375601895.1 YggT family protein -
  AB9X52_RS18385 (AB9X52_18380) yggU 3854297..3854593 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  AB9X52_RS18390 (AB9X52_18385) - 3854597..3855190 (+) 594 WP_148385179.1 XTP/dITP diphosphatase -
  AB9X52_RS18395 (AB9X52_18390) hemW 3855183..3856331 (+) 1149 WP_375601896.1 radical SAM family heme chaperone HemW -
  AB9X52_RS18400 (AB9X52_18395) - 3856388..3856750 (+) 363 WP_074135713.1 endonuclease domain-containing protein -
  AB9X52_RS18405 (AB9X52_18400) - 3856864..3857580 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  AB9X52_RS18410 (AB9X52_18405) - 3857638..3857964 (-) 327 WP_148385183.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35666.70 Da        Isoelectric Point: 6.3047

>NTDB_id=1034448 AB9X52_RS18370 WP_375601894.1 3852013..3852993(-) (pilT) [Enterobacter hormaechei strain 2024CK-00699]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMFQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1034448 AB9X52_RS18370 WP_375601894.1 3852013..3852993(-) (pilT) [Enterobacter hormaechei strain 2024CK-00699]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAACAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCGGTTACTCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTACCGCGAGCGATCCCGGAACTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACACTGGAAGATCCGGTGGAG
TTTATGTTCCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATTGGCCAGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment