Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8W27_RS03515 Genome accession   NZ_CP165997
Coordinates   730768..731784 (+) Length   338 a.a.
NCBI ID   WP_015742431.1    Uniprot ID   -
Organism   Cronobacter turicensis strain LMG23827     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725768..736784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8W27_RS03485 (AB8W27_03485) ftsP 725856..727268 (+) 1413 WP_007763988.1 cell division protein FtsP -
  AB8W27_RS03490 (AB8W27_03490) hemW 727446..728582 (-) 1137 WP_015742434.1 radical SAM family heme chaperone HemW -
  AB8W27_RS03495 (AB8W27_03495) - 728575..729168 (-) 594 WP_041923912.1 XTP/dITP diphosphatase -
  AB8W27_RS03500 (AB8W27_03500) yggU 729183..729473 (-) 291 WP_004385624.1 DUF167 family protein YggU -
  AB8W27_RS03505 (AB8W27_03505) - 729470..730024 (-) 555 WP_007763984.1 YggT family protein -
  AB8W27_RS03510 (AB8W27_03510) - 730040..730753 (-) 714 WP_041923911.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8W27_RS03515 (AB8W27_03515) pilT 730768..731784 (+) 1017 WP_015742431.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8W27_RS03520 (AB8W27_03520) - 731781..734039 (-) 2259 WP_041923910.1 FAD-dependent oxidoreductase -
  AB8W27_RS03525 (AB8W27_03525) - 734102..734794 (+) 693 WP_015742429.1 IclR family transcriptional regulator -
  AB8W27_RS03530 (AB8W27_03530) ruvX 734955..735371 (-) 417 WP_015742428.1 Holliday junction resolvase RuvX -
  AB8W27_RS03535 (AB8W27_03535) - 735371..735934 (-) 564 WP_041923909.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36710.20 Da        Isoelectric Point: 6.4258

>NTDB_id=1032853 AB8W27_RS03515 WP_015742431.1 730768..731784(+) (pilT) [Cronobacter turicensis strain LMG23827]
MEMDELVALSVKHNVSDLHLCSELPARWRRQGRLEPLPDAVPKPENLLARWLDDAHQRELAERGQTDFAVSLAGGVRLRG
NAFRQMQGVSLVLRLLPRGCPRLEALGVPAAVPELLTMPDGLMLVTGATGSGKSTTLAAMVGYLNETLDGHILTLEDPVE
FCHQPARCLIQQREIGTHCQSFSDGLRGALREDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPADEQNKVRSQLAGSLKAVLAQKLVPDVQGGRTALHELLINTPAVANLIREGKTHQLPGLMETGQQQGMQTFAQSVAMR
LNEGKLALQKDASAGHWC

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1032853 AB8W27_RS03515 WP_015742431.1 730768..731784(+) (pilT) [Cronobacter turicensis strain LMG23827]
ATGGAAATGGATGAATTAGTGGCGCTTAGTGTAAAGCATAACGTTTCGGATCTACACCTGTGTAGCGAGCTACCGGCGCG
CTGGCGCAGGCAGGGGCGGCTGGAGCCGCTGCCTGATGCCGTCCCGAAGCCGGAGAACCTGCTGGCGCGCTGGCTCGACG
ACGCCCATCAGCGCGAGCTTGCCGAACGGGGGCAGACCGATTTCGCAGTTAGTCTTGCAGGCGGCGTGCGGCTGCGCGGC
AATGCGTTTCGCCAGATGCAGGGGGTGTCGCTGGTATTGCGCCTGCTGCCGCGAGGCTGCCCCAGGCTTGAAGCGCTCGG
CGTGCCCGCAGCGGTGCCAGAGCTATTGACGATGCCGGATGGCCTGATGCTGGTCACAGGGGCGACCGGCAGCGGCAAAT
CGACGACGCTCGCCGCGATGGTCGGCTACCTGAATGAGACGCTCGACGGCCATATTCTGACGCTTGAAGATCCGGTCGAG
TTTTGTCATCAACCGGCGCGCTGTCTTATCCAGCAGCGTGAAATCGGCACGCACTGCCAAAGCTTCAGCGACGGCCTGCG
CGGCGCGCTGCGTGAAGATCCGGATGTCATTTTGCTGGGGGAGCTGCGCGACAGCGAAACCATCCGGCTGGCGCTTACGG
CGGCGGAGACCGGCCATCTGGTGATGGCGACGCTGCATACGCGCGGCGCGGCGCAGGCCGTCGAGCGGCTGGTGGATGTC
TTTCCTGCTGATGAACAGAATAAAGTCCGCAGCCAGTTGGCGGGCAGCCTGAAAGCGGTGCTGGCGCAAAAGCTGGTGCC
GGACGTTCAGGGCGGCAGAACGGCGCTGCATGAACTTCTCATCAATACGCCCGCCGTGGCGAATCTTATCCGCGAGGGGA
AAACGCACCAGCTGCCGGGCCTGATGGAAACCGGCCAGCAGCAGGGGATGCAAACCTTCGCGCAAAGCGTGGCGATGCGT
CTGAACGAAGGCAAACTGGCGCTGCAAAAAGACGCCAGCGCCGGGCACTGGTGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.657

99.112

0.482
  pilT Vibrio cholerae strain A1552

48.657

99.112

0.482
  pilT Neisseria meningitidis 8013

49.096

98.225

0.482
  pilT Neisseria gonorrhoeae MS11

48.494

98.225

0.476
  pilT Pseudomonas stutzeri DSM 10701

50.316

93.491

0.47
  pilT Acinetobacter baylyi ADP1

47.59

98.225

0.467
  pilT Acinetobacter baumannii D1279779

45.588

100

0.459
  pilT Acinetobacter nosocomialis M2

45.588

100

0.459
  pilT Acinetobacter baumannii strain A118

45.588

100

0.459
  pilT Pseudomonas aeruginosa PAK

48.734

93.491

0.456
  pilT Legionella pneumophila strain ERS1305867

46.084

98.225

0.453
  pilT Legionella pneumophila strain Lp02

46.084

98.225

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.827

95.858

0.42
  pilU Pseudomonas stutzeri DSM 10701

41.742

98.521

0.411
  pilU Vibrio cholerae strain A1552

40.964

98.225

0.402

Multiple sequence alignment