Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB4O82_RS18025 Genome accession   NZ_CP163086
Coordinates   3795684..3796664 (-) Length   326 a.a.
NCBI ID   WP_276170183.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain W76     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3790684..3801664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4O82_RS17995 (AB4O82_17995) - 3791561..3792058 (+) 498 WP_023298567.1 SprT family zinc-dependent metalloprotease -
  AB4O82_RS18000 (AB4O82_18000) endA 3792153..3792860 (+) 708 WP_022649352.1 deoxyribonuclease I -
  AB4O82_RS18005 (AB4O82_18005) rsmE 3792912..3793643 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB4O82_RS18010 (AB4O82_18010) gshB 3793675..3794622 (+) 948 WP_003860032.1 glutathione synthase -
  AB4O82_RS18015 (AB4O82_18015) - 3794697..3795257 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  AB4O82_RS18020 (AB4O82_18020) ruvX 3795257..3795673 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  AB4O82_RS18025 (AB4O82_18025) pilT 3795684..3796664 (-) 981 WP_276170183.1 type IV pilus twitching motility protein PilT Machinery gene
  AB4O82_RS18030 (AB4O82_18030) - 3796682..3797383 (+) 702 WP_171932611.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB4O82_RS18035 (AB4O82_18035) - 3797405..3797971 (+) 567 WP_003860024.1 YggT family protein -
  AB4O82_RS18040 (AB4O82_18040) yggU 3797968..3798264 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  AB4O82_RS18045 (AB4O82_18045) - 3798268..3798861 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  AB4O82_RS18050 (AB4O82_18050) hemW 3798854..3800002 (+) 1149 WP_022649358.1 radical SAM family heme chaperone HemW -
  AB4O82_RS18055 (AB4O82_18055) - 3800059..3800397 (+) 339 WP_023298571.1 endonuclease domain-containing protein -
  AB4O82_RS18060 (AB4O82_18060) - 3800481..3801197 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  AB4O82_RS18065 (AB4O82_18065) - 3801255..3801581 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35703.68 Da        Isoelectric Point: 6.6603

>NTDB_id=1028728 AB4O82_RS18025 WP_276170183.1 3795684..3796664(-) (pilT) [Enterobacter hormaechei strain W76]
MDVEEIVTLSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSQRCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1028728 AB4O82_RS18025 WP_276170183.1 3795684..3796664(-) (pilT) [Enterobacter hormaechei strain W76]
ATGGATGTGGAAGAAATTGTGACCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGTGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTCAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment