Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB3N44_RS16870 Genome accession   NZ_CP162195
Coordinates   3532298..3533278 (+) Length   326 a.a.
NCBI ID   WP_252002313.1    Uniprot ID   -
Organism   Enterobacter chuandaensis strain STK53-E     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3527298..3538278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N44_RS16830 (AB3N44_16820) - 3527381..3527707 (+) 327 WP_003862421.1 YggL family protein -
  AB3N44_RS16835 (AB3N44_16825) - 3527765..3528481 (+) 717 WP_080330040.1 DUF2884 domain-containing protein -
  AB3N44_RS16840 (AB3N44_16830) - 3528564..3528902 (-) 339 WP_119917080.1 endonuclease domain-containing protein -
  AB3N44_RS16845 (AB3N44_16835) hemW 3528966..3530108 (-) 1143 WP_333016988.1 radical SAM family heme chaperone HemW -
  AB3N44_RS16850 (AB3N44_16840) - 3530101..3530694 (-) 594 WP_048979374.1 XTP/dITP diphosphatase -
  AB3N44_RS16855 (AB3N44_16845) yggU 3530698..3530994 (-) 297 WP_333016989.1 DUF167 family protein YggU -
  AB3N44_RS16860 (AB3N44_16850) - 3530991..3531557 (-) 567 WP_080330042.1 YggT family protein -
  AB3N44_RS16865 (AB3N44_16855) - 3531579..3532280 (-) 702 WP_080330043.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3N44_RS16870 (AB3N44_16860) pilT 3532298..3533278 (+) 981 WP_252002313.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3N44_RS16875 (AB3N44_16865) ruvX 3533289..3533705 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  AB3N44_RS16880 (AB3N44_16870) - 3533705..3534265 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  AB3N44_RS16885 (AB3N44_16875) gshB 3534340..3535287 (-) 948 WP_033146605.1 glutathione synthase -
  AB3N44_RS16890 (AB3N44_16880) rsmE 3535307..3536038 (-) 732 WP_333016990.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB3N44_RS16895 (AB3N44_16885) endA 3536090..3536797 (-) 708 WP_406808115.1 deoxyribonuclease I -
  AB3N44_RS16900 (AB3N44_16890) - 3536892..3537389 (-) 498 WP_217480269.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35493.53 Da        Isoelectric Point: 6.4994

>NTDB_id=1024614 AB3N44_RS16870 WP_252002313.1 3532298..3533278(+) (pilT) [Enterobacter chuandaensis strain STK53-E]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLESAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGNQRLRG
SAFKQMKGVSVTLRLLPRSCPQLASLGVPRALPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1024614 AB3N44_RS16870 WP_252002313.1 3532298..3533278(+) (pilT) [Enterobacter chuandaensis strain STK53-E]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGACTAGGCCGCCTTGAATCGGCCCCGTTTCCGCCGCCTGATGTCGGGGCGTTATTAAAGGCGTGGCTGAACG
ACGAGCAGCAGGGAGCCTGGTGGGCAAACGGACAGGTCGATTTTGCCGTGACGGTCGCAGGAAACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCGTCTCCGTTACCCTGCGCCTGTTGCCGCGTTCCTGTCCGCAGCTCGCTTCGCTGGG
TGTGCCGCGGGCGCTTCCCGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCCACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCGATGGTCGATTTTCTCAACCGCCAGACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGCGAGATTGGGCAACACAGCCCGTCCTTTGCCGAGGCGCTGCG
CGGCGCATTACGCGAGGATCCGGACGTTATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGTGGGGCAGCGCAGGCAATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTCCAGGGCGGGCGCGTCGCGCTGTATGAACTGTTGGTGAACACCGCTGCTGCGGCGAATCTGATTCGTGAAGGGA
AGACGTGGCAACTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39

Multiple sequence alignment