Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB0U89_RS03820 Genome accession   NZ_CP160167
Coordinates   785781..786761 (+) Length   326 a.a.
NCBI ID   WP_023202164.1    Uniprot ID   A0A5I4NRB9
Organism   Salmonella enterica subsp. enterica strain N23-0176     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 780781..791761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0U89_RS03790 (AB0U89_03790) - 781359..782366 (+) 1008 WP_023254048.1 DUF1202 domain-containing protein -
  AB0U89_RS03795 (AB0U89_03795) hemW 782457..783593 (-) 1137 WP_023254049.1 radical SAM family heme chaperone HemW -
  AB0U89_RS03800 (AB0U89_03800) - 783586..784179 (-) 594 WP_023254050.1 XTP/dITP diphosphatase -
  AB0U89_RS03805 (AB0U89_03805) yggU 784187..784477 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  AB0U89_RS03810 (AB0U89_03810) - 784474..785040 (-) 567 WP_001094848.1 YggT family protein -
  AB0U89_RS03815 (AB0U89_03815) - 785059..785763 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB0U89_RS03820 (AB0U89_03820) pilT 785781..786761 (+) 981 WP_023202164.1 type IV pilus twitching motility protein PilT Machinery gene
  AB0U89_RS03825 (AB0U89_03825) - 786890..787576 (+) 687 WP_000098329.1 IclR family transcriptional regulator -
  AB0U89_RS03830 (AB0U89_03830) ruvX 787623..788039 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  AB0U89_RS03835 (AB0U89_03835) - 788039..788602 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  AB0U89_RS03840 (AB0U89_03840) gshB 788818..789765 (-) 948 WP_023232636.1 glutathione synthase -
  AB0U89_RS03845 (AB0U89_03845) rsmE 789785..790516 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB0U89_RS03850 (AB0U89_03850) endA 790593..791300 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36124.34 Da        Isoelectric Point: 7.5355

>NTDB_id=1018559 AB0U89_RS03820 WP_023202164.1 785781..786761(+) (pilT) [Salmonella enterica subsp. enterica strain N23-0176]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1018559 AB0U89_RS03820 WP_023202164.1 785781..786761(+) (pilT) [Salmonella enterica subsp. enterica strain N23-0176]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5I4NRB9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.336

100

0.371

Multiple sequence alignment