Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   N027_RS04080 Genome accession   NZ_CP159278
Coordinates   851168..851599 (+) Length   143 a.a.
NCBI ID   WP_024659293.1    Uniprot ID   A0AAU8MCN4
Organism   Pseudomonas syringae USA007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 846168..856599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS04055 (N027_04055) yacG 846326..846535 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  N027_RS04060 (N027_04060) coaE 846532..847155 (-) 624 WP_024659289.1 dephospho-CoA kinase -
  N027_RS04065 (N027_04065) pilD 847152..848024 (-) 873 WP_024659290.1 A24 family peptidase Machinery gene
  N027_RS04070 (N027_04070) pilC 848026..849243 (-) 1218 WP_024659291.1 type II secretion system F family protein Machinery gene
  N027_RS04075 (N027_04075) pilB 849246..850940 (-) 1695 WP_024659292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N027_RS04080 (N027_04080) pilA2 851168..851599 (+) 432 WP_024659293.1 pilin Machinery gene
  N027_RS04085 (N027_04085) - 851889..852239 (-) 351 WP_024659294.1 helix-turn-helix domain-containing protein -
  N027_RS04090 (N027_04090) gstA 852396..853016 (+) 621 WP_024659295.1 glutathione transferase GstA -
  N027_RS04095 (N027_04095) - 853115..853660 (-) 546 WP_024659296.1 DUF1523 family protein -
  N027_RS04100 (N027_04100) - 853781..854779 (+) 999 WP_024659297.1 ATP-grasp domain-containing protein -
  N027_RS04105 (N027_04105) - 854834..855982 (+) 1149 WP_024659298.1 trypsin-like peptidase domain-containing protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14579.77 Da        Isoelectric Point: 9.1226

>NTDB_id=1013008 N027_RS04080 WP_024659293.1 851168..851599(+) (pilA2) [Pseudomonas syringae USA007]
MKSQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRSKVSEGLAQASSAKLAVAETAANVGGIANVTAANSGFVFNQVAT
NNSYVASVVVGAGGVITVVTKATGATDQPAFQLVPAQASVNDQITWTCTRTAGLAKHLPANCR

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1013008 N027_RS04080 WP_024659293.1 851168..851599(+) (pilA2) [Pseudomonas syringae USA007]
ATGAAATCACAAAAAGGTTTTACATTGATCGAACTGATGATCGTTGTAGCGATTATTGGTATTTTGGCGGCGGTTGCGCT
GCCTGCCTATCAGGATTACACCATCAGAAGCAAGGTCAGTGAAGGCTTGGCGCAAGCGAGCTCCGCGAAGCTAGCAGTTG
CTGAGACGGCCGCTAACGTTGGCGGTATTGCAAACGTAACAGCAGCTAACTCCGGCTTTGTGTTTAACCAAGTGGCTACG
AATAACTCGTATGTGGCATCTGTTGTGGTCGGTGCTGGCGGCGTCATCACTGTTGTGACCAAAGCTACCGGTGCAACAGA
TCAGCCAGCGTTTCAGCTCGTCCCGGCACAAGCTTCAGTCAACGATCAGATCACTTGGACTTGTACAAGGACTGCTGGTC
TTGCCAAGCACCTTCCTGCTAACTGCCGGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

54.861

100

0.552
  pilA2 Legionella pneumophila strain ERS1305867

54.167

100

0.545
  comP Acinetobacter baylyi ADP1

45.752

100

0.49
  pilA Ralstonia pseudosolanacearum GMI1000

40.351

100

0.483
  pilE Neisseria gonorrhoeae strain FA1090

42.038

100

0.462
  pilE Neisseria gonorrhoeae MS11

39.873

100

0.441
  pilA/pilA1 Eikenella corrodens VA1

39.735

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

97.902

0.392
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.286

97.902

0.385
  pilA Haemophilus influenzae Rd KW20

39.13

96.503

0.378
  pilA Haemophilus influenzae 86-028NP

38.406

96.503

0.371
  pilA Glaesserella parasuis strain SC1401

37.857

97.902

0.371

Multiple sequence alignment