Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N027_RS04070 Genome accession   NZ_CP159278
Coordinates   848026..849243 (-) Length   405 a.a.
NCBI ID   WP_024659291.1    Uniprot ID   A0AAU8MBC4
Organism   Pseudomonas syringae USA007     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 843026..854243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS04035 (N027_04035) - 843098..843727 (-) 630 WP_005736090.1 DUF1780 domain-containing protein -
  N027_RS04040 (N027_04040) - 843842..844156 (+) 315 WP_235202550.1 hypothetical protein -
  N027_RS04045 (N027_04045) - 844153..845625 (+) 1473 WP_024659288.1 tetratricopeptide repeat protein -
  N027_RS04050 (N027_04050) - 845723..846187 (+) 465 WP_354675731.1 hypothetical protein -
  N027_RS04055 (N027_04055) yacG 846326..846535 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  N027_RS04060 (N027_04060) coaE 846532..847155 (-) 624 WP_024659289.1 dephospho-CoA kinase -
  N027_RS04065 (N027_04065) pilD 847152..848024 (-) 873 WP_024659290.1 A24 family peptidase Machinery gene
  N027_RS04070 (N027_04070) pilC 848026..849243 (-) 1218 WP_024659291.1 type II secretion system F family protein Machinery gene
  N027_RS04075 (N027_04075) pilB 849246..850940 (-) 1695 WP_024659292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N027_RS04080 (N027_04080) pilA2 851168..851599 (+) 432 WP_024659293.1 pilin Machinery gene
  N027_RS04085 (N027_04085) - 851889..852239 (-) 351 WP_024659294.1 helix-turn-helix domain-containing protein -
  N027_RS04090 (N027_04090) gstA 852396..853016 (+) 621 WP_024659295.1 glutathione transferase GstA -
  N027_RS04095 (N027_04095) - 853115..853660 (-) 546 WP_024659296.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44003.92 Da        Isoelectric Point: 10.1287

>NTDB_id=1013006 N027_RS04070 WP_024659291.1 848026..849243(-) (pilC) [Pseudomonas syringae USA007]
MASKTVKLSVYTWEGVDKKGGKLSGELSGHNPALIKAQLRKQGVNPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPMFTLMVIGLSEVIQQWWIAIVVAFFAGFFLFK
KAYKKSQRFRDSIDRLLLKIPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRATGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAILGVVVGGLVIAMYLPIFK
LGDVA

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1013006 N027_RS04070 WP_024659291.1 848026..849243(-) (pilC) [Pseudomonas syringae USA007]
ATGGCAAGCAAGACAGTCAAACTCAGTGTCTACACTTGGGAAGGCGTAGACAAAAAAGGTGGCAAACTCAGTGGGGAATT
GAGCGGACACAACCCGGCGCTCATTAAGGCACAATTACGTAAACAGGGCGTAAACCCTACCAAGGTGCGCAAAAAATCTG
TTTCGATTTTTGGTAAAGGCAAAAAGATAAAACCGCTGGATATTGCCTTTTTCTCTCGACAGATGGCGACAATGATGAAA
GCTGGCGTGCCGCTGCTACAGTCGTTCGACATCATTAGCGAAGGGGCAGAAAATCCGAACATGCGAGCATTGGTAAACTC
GCTCAAGCAAGAAGTTTCGGCGGGAAACAGCTTCGCAACAGCGTTACGGCAAAAGCCTGAGTATTTTGATGACCTGTTTT
GTAATCTAGTGGACGCAGGGGAGCAGGCAGGTGCACTTGAGAGCTTGCTGGACCGCGTCGCGACTTATAAAGAAAAAACC
GAAAAACTCAAAGCCAAGATCAAGAAAGCTATGACCTATCCTGCTGCGGTGTTAATCGTTGCAGTCATCGTATCAGGTAT
TCTACTCATAAAAGTCGTACCGCAATTCCAGTCGGTTTTTGCAGGTTTTGGTGCCGAGCTACCAATGTTCACATTGATGG
TAATCGGACTTTCTGAGGTCATCCAGCAATGGTGGATAGCTATTGTCGTAGCATTCTTTGCGGGTTTTTTCTTGTTCAAA
AAGGCTTATAAAAAATCACAGAGATTTCGAGACAGCATTGACCGACTCTTATTGAAGATTCCGGTCATTGGACCTTTGAT
TTTCAAGTCTTCAGTTGCACGTTATGCTCGGACGCTGGCGACCACATTCGCGGCCGGTGTCCCGCTAGTGGAGGCGTTAG
ATTCGGTCGCTGGCGCGACTGGCAATGTGGTTTTTAAAAATGCAGTAAACAAGGTAAAACAAGACGTGTCCACAGGGATG
CAGTTGAACTTTTCAATGCGCGCGACGGGGGTTTTCCCGAGCTTGGCAATTCAGATGACTGCGATTGGCGAGGAATCCGG
GGCTCTGGACAACATGTTGGATAAGGTTGCGACATATTACGAGGATGAAGTTGATAATATGGTCGATAGCCTTACCAGCC
TCATGGAGCCAATGATCATGGCCATACTCGGTGTTGTTGTTGGCGGTTTGGTGATTGCAATGTACTTGCCAATATTCAAA
CTGGGAGACGTCGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.257

99.753

0.741
  pilC Acinetobacter baylyi ADP1

59.753

100

0.598
  pilC Acinetobacter baumannii D1279779

59.649

98.519

0.588
  pilC Legionella pneumophila strain ERS1305867

52.109

99.506

0.519
  pilG Neisseria gonorrhoeae MS11

43.467

98.272

0.427
  pilC Vibrio cholerae strain A1552

42.327

99.753

0.422
  pilG Neisseria meningitidis 44/76-A

42.424

97.778

0.415
  pilC Vibrio campbellii strain DS40M4

41.604

98.519

0.41
  pilC Thermus thermophilus HB27

38.958

99.506

0.388

Multiple sequence alignment