Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N027_RS04075 Genome accession   NZ_CP159278
Coordinates   849246..850940 (-) Length   564 a.a.
NCBI ID   WP_024659292.1    Uniprot ID   A0AAU8MBX6
Organism   Pseudomonas syringae USA007     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 844246..855940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N027_RS04050 (N027_04050) - 845723..846187 (+) 465 WP_354675731.1 hypothetical protein -
  N027_RS04055 (N027_04055) yacG 846326..846535 (-) 210 WP_003381207.1 DNA gyrase inhibitor YacG -
  N027_RS04060 (N027_04060) coaE 846532..847155 (-) 624 WP_024659289.1 dephospho-CoA kinase -
  N027_RS04065 (N027_04065) pilD 847152..848024 (-) 873 WP_024659290.1 A24 family peptidase Machinery gene
  N027_RS04070 (N027_04070) pilC 848026..849243 (-) 1218 WP_024659291.1 type II secretion system F family protein Machinery gene
  N027_RS04075 (N027_04075) pilB 849246..850940 (-) 1695 WP_024659292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N027_RS04080 (N027_04080) pilA2 851168..851599 (+) 432 WP_024659293.1 pilin Machinery gene
  N027_RS04085 (N027_04085) - 851889..852239 (-) 351 WP_024659294.1 helix-turn-helix domain-containing protein -
  N027_RS04090 (N027_04090) gstA 852396..853016 (+) 621 WP_024659295.1 glutathione transferase GstA -
  N027_RS04095 (N027_04095) - 853115..853660 (-) 546 WP_024659296.1 DUF1523 family protein -
  N027_RS04100 (N027_04100) - 853781..854779 (+) 999 WP_024659297.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62461.65 Da        Isoelectric Point: 6.3854

>NTDB_id=1013007 N027_RS04075 WP_024659292.1 849246..850940(-) (pilB) [Pseudomonas syringae USA007]
MTDVVLSGLAKQLVLAELLTETAAQQAYQQARRDKISLVSYLVQNKLVKSLTLAEMASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHTLPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTAAIDRFFDSDSGLGNLEDVDLGLD
IEPSDGKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKTFRVRLRTDGILHEVAKPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIREGFPESK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1013007 N027_RS04075 WP_024659292.1 849246..850940(-) (pilB) [Pseudomonas syringae USA007]
ATGACTGATGTTGTTCTTTCTGGCTTGGCCAAACAGCTTGTACTGGCCGAGCTACTCACTGAAACTGCTGCGCAGCAAGC
CTATCAACAGGCGCGCCGTGACAAGATCTCGTTAGTCAGCTATTTAGTTCAGAATAAACTGGTCAAAAGTCTGACGCTCG
CAGAAATGGCATCGGACCAGTTCGGCGTGCCGTTCATGGACCTAGCCAGCCTGGACAAGGAAAGCCAGCCCAAAGGACTT
GTTAGCGAGAAGCTTGTTCGACAGCATCACACGCTTCCCCTGTGGCGACGCGGTAACAAGCTGTTCATAGGCATTTCAGA
CCCCACTAATCATCAGGCAGTCACCGACATTCAGTTCAGCACCGGCCTGAATACCGAAGCCATTCTGGTCGAAGATGACA
AGCTGACAGCTGCAATAGATCGTTTTTTTGACAGCGATAGCGGTTTGGGCAATCTGGAAGATGTCGACCTGGGCCTGGAT
ATCGAACCGTCTGATGGCAAGGAAACATCTCTTGCTACTCAGAATGACGCTGACGACGCCCCAGTGGTACGCTTTGTAAA
CAAGATGTTGATGGACGCAATTCGTCTTGGCTCGTCAGACCTGCACTTTGAACCCTACGAAAAAACATTCCGTGTGCGCT
TGCGTACCGACGGCATTTTGCATGAAGTCGCAAAGCCACCAATCCACTTGGCAAATCGTATAGCTGCACGTCTGAAGGTC
ATGGCCAGCCTCGATATATCTGAGCGGCGCAAACCGCAGGACGGACGCGTCAAGTTGCGGGTGTCCAAAACAAAAGCAAT
CGATTTTCGTATGAATACCCTGCCCACATTGTGGGGCGAGAAGATCGTGATGCGAATTCTCGACCCGACCAGTGCGCAGA
TGGGCATTGACGCTCTGGGCTACGAGCCGGAACAAAAAGCACTGTATCTGGAGGCCCTGAAGCAGCCGCAGGGCATGATC
CTTGTGACTGGCCCGACTGGTTCCGGCAAGACCGTTTCTCTGTATACCGGTCTGAATATCCTCAATACAGTGGACATCAA
CATTTCCACAGCCGAAGATCCGGTAGAGATCAATCTGGAAGGCATCAATCAGGTCAACGTCAACCCACGCCAAGGATTGG
ACTTTTCGCAGGCATTGCGCGCCTTCCTGCGTCAAGATCCCGACGTGATCATGGTCGGCGAGATTCGCGACCTGGAAACC
GCCGAAATCGCCATCAAGGCGTCACAGACCGGCCACATGGTGTTGTCCACCCTGCACACCAACAGCGCGGCAGAAACCTT
GACGCGACTGCATCATATGGGTGTCGCAGCATTCAACATTGCTACAGCAATCAACCTGATTATTGCGCAACGGCTTGCGC
GCAAGCTGTGCAGCCATTGCAAGAAAGAGCTCGACATCCCTCGTGAGACGCTGATTCGGGAAGGCTTTCCAGAGTCAAAA
ATAGGCACTTTCAAAATCTATGGGCCGGTGGGCTGCGAGCACTGCAACGGTGGTTACAGAGGCAGGGTCGGTATCTACGA
AGTCGTCAAGAAAACAGCAGAGCTGGAACGTATCATCATGGAAGAAGGCAATTCGCTTGAAATCTCCAGGCAGATGCGCA
AAGACGGCTTCAATGATCTGCGAACCTCGGGTCTGTCAAAAGCCATGCAGGGCATTACCAGCCTGGAAGAAGTCAACCGC
GTTACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.726

99.823

0.576
  pilB Acinetobacter baylyi ADP1

56.94

99.645

0.567
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

50.89

99.645

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.09

98.759

0.495
  pilB Vibrio campbellii strain DS40M4

48.94

100

0.491
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

38.869

100

0.39

Multiple sequence alignment