Detailed information NTG1013005

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: N027_RS04065
• Genome accession: NZ_CP159278
• Coordinates: 847152..848024 (-)
• Length: 290 a.a.
• NCBI ID: WP_024659290.1
• Uniprot ID: -
• Organism: Pseudomonas syringae USA007
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 842152..853024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N027_RS04030 (N027_04030)	-	842568..843101 (-)	534	WP_024659286.1	MOSC domain-containing protein	-
N027_RS04035 (N027_04035)	-	843098..843727 (-)	630	WP_005736090.1	DUF1780 domain-containing protein	-
N027_RS04040 (N027_04040)	-	843842..844156 (+)	315	WP_235202550.1	hypothetical protein	-
N027_RS04045 (N027_04045)	-	844153..845625 (+)	1473	WP_024659288.1	tetratricopeptide repeat protein	-
N027_RS04050 (N027_04050)	-	845723..846187 (+)	465	WP_354675731.1	hypothetical protein	-
N027_RS04055 (N027_04055)	yacG	846326..846535 (-)	210	WP_003381207.1	DNA gyrase inhibitor YacG	-
N027_RS04060 (N027_04060)	coaE	846532..847155 (-)	624	WP_024659289.1	dephospho-CoA kinase	-
N027_RS04065 (N027_04065)	pilD	847152..848024 (-)	873	WP_024659290.1	A24 family peptidase	Machinery gene
N027_RS04070 (N027_04070)	pilC	848026..849243 (-)	1218	WP_024659291.1	type II secretion system F family protein	Machinery gene
N027_RS04075 (N027_04075)	pilB	849246..850940 (-)	1695	WP_024659292.1	type IV-A pilus assembly ATPase PilB	Machinery gene
N027_RS04080 (N027_04080)	pilA2	851168..851599 (+)	432	WP_024659293.1	pilin	Machinery gene
N027_RS04085 (N027_04085)	-	851889..852239 (-)	351	WP_024659294.1	helix-turn-helix domain-containing protein	-
N027_RS04090 (N027_04090)	gstA	852396..853016 (+)	621	WP_024659295.1	glutathione transferase GstA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32009.24 Da
• Isoelectric Point: 8.1553

>NTDB_id=1013005 N027_RS04065 WP_024659290.1 847152..848024(-) (pilD) [Pseudomonas syringae USA007]
MPLLDLLASSPLAFVTTCCLLGLIVGSFLNVVVYRLPKMMERDWKAQSREMLGLPAEPDQPVFNLILPHSSCPHCAHKIR
PWENLPIISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLTLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=1013005 N027_RS04065 WP_024659290.1 847152..848024(-) (pilD) [Pseudomonas syringae USA007]
ATGCCCCTCCTCGACCTCTTGGCAAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCCTGCTCGGCCTAATCGTCGGAAG
CTTCCTCAACGTAGTCGTCTACCGCCTCCCAAAAATGATGGAGCGCGACTGGAAAGCGCAATCACGGGAAATGCTCGGCC
TCCCTGCCGAACCGGATCAACCCGTCTTCAACCTGATCCTGCCGCACTCCAGTTGCCCGCACTGCGCGCACAAGATACGC
CCGTGGGAAAACCTGCCGATCATCAGTTACCTGTTGCTGCGGGGCAAATGCTCACAATGCAAAGCCCCGATCAGCAAGCG
CTACCCATTGGTGGAGCTGACCTGCGCAGTGTTATCGGCCTACGTCGCCTGGCATTTCGGCTTTGGCTGGCAGGCGGCTG
CGATGCTGGTGTTGAGTTGGGGTTTGCTGGCGATGAGCCTGATCGATGCCGATCATCAGCTGTTGCCTGACTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTTAACGCCTTTGGGCTGTTCACCTCGCTGAATGATGCGTTGTGGGGTGCGGT
GGCCGGTTATCTGACGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCTATGGCGACT
TCAAATTGCTGGCCATGCTTGGCGCGTGGGGTGGCTGGCAGATTCTGCCGTTGACCATTCTGTTGTCGTCGCTGGTGGGT
GCGGTACTTGGGGTGATCATGATGCGCGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTATATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.417	97.586	0.531
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.555	94.483	0.497
pilD	Acinetobacter nosocomialis M2	52.19	94.483	0.493
pilD	Neisseria gonorrhoeae MS11	52.239	92.414	0.483