Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AASJ52_RS11755 Genome accession   NZ_CP156168
Coordinates   2479993..2480973 (+) Length   326 a.a.
NCBI ID   WP_001055655.1    Uniprot ID   A0A5Z0RZB6
Organism   Salmonella enterica subsp. enterica serovar Paratyphi A strain 45157     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474993..2485973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AASJ52_RS11725 (AASJ52_11725) - 2475571..2476578 (+) 1008 WP_000252189.1 DUF1202 domain-containing protein -
  AASJ52_RS11730 (AASJ52_11730) hemW 2476669..2477805 (-) 1137 WP_001096530.1 radical SAM family heme chaperone HemW -
  AASJ52_RS11735 (AASJ52_11735) - 2477798..2478391 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  AASJ52_RS11740 (AASJ52_11740) yggU 2478399..2478689 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  AASJ52_RS11745 (AASJ52_11745) - 2478686..2479252 (-) 567 WP_001094848.1 YggT family protein -
  AASJ52_RS11750 (AASJ52_11750) - 2479271..2479975 (-) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  AASJ52_RS11755 (AASJ52_11755) pilT 2479993..2480973 (+) 981 WP_001055655.1 type IV pilus twitching motility protein PilT Machinery gene
  AASJ52_RS11760 (AASJ52_11760) - 2481105..2481791 (+) 687 WP_000098319.1 IclR family transcriptional regulator -
  AASJ52_RS11765 (AASJ52_11765) ruvX 2481838..2482254 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  AASJ52_RS11770 (AASJ52_11770) - 2482254..2482817 (-) 564 WP_001053176.1 YqgE/AlgH family protein -
  AASJ52_RS11775 (AASJ52_11775) gshB 2483033..2483980 (-) 948 WP_000593248.1 glutathione synthase -
  AASJ52_RS11780 (AASJ52_11780) rsmE 2484000..2484731 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AASJ52_RS11785 (AASJ52_11785) endA 2484808..2485515 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36139.35 Da        Isoelectric Point: 7.8164

>NTDB_id=1001711 AASJ52_RS11755 WP_001055655.1 2479993..2480973(+) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A strain 45157]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFKHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREVGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1001711 AASJ52_RS11755 WP_001055655.1 2479993..2480973(+) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A strain 45157]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCAAACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAGTCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z0RZB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment