Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABFU31_RS16320 Genome accession   NZ_CP155950
Coordinates   3732452..3732850 (-) Length   132 a.a.
NCBI ID   WP_104570927.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain bglFP 6814     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3721348..3745195 3732452..3732850 within 0


Gene organization within MGE regions


Location: 3721348..3745195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU31_RS16275 (ABFU31_16260) pilB 3722333..3724069 (-) 1737 WP_040940802.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU31_RS16280 (ABFU31_16265) - 3724225..3725076 (+) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  ABFU31_RS16285 (ABFU31_16270) - 3725126..3725989 (+) 864 WP_116921534.1 glycosyltransferase -
  ABFU31_RS16290 (ABFU31_16275) - 3725974..3727146 (+) 1173 WP_181920810.1 glycosyltransferase -
  ABFU31_RS16295 (ABFU31_16280) - 3727140..3727982 (+) 843 WP_147308817.1 glycosyltransferase -
  ABFU31_RS16300 (ABFU31_16285) - 3727985..3728830 (-) 846 WP_323535832.1 glycosyltransferase family 9 protein -
  ABFU31_RS16305 (ABFU31_16290) - 3729168..3729926 (-) 759 WP_116921530.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  ABFU31_RS16310 (ABFU31_16295) - 3730050..3731870 (-) 1821 WP_147308815.1 hypothetical protein -
  ABFU31_RS16315 (ABFU31_16300) - 3731929..3732351 (-) 423 WP_104570926.1 pilin -
  ABFU31_RS16320 (ABFU31_16305) pilA/pilAI 3732452..3732850 (-) 399 WP_104570927.1 pilin Machinery gene
  ABFU31_RS16325 (ABFU31_16310) pilC 3733201..3734463 (+) 1263 WP_104570928.1 type II secretion system F family protein Machinery gene
  ABFU31_RS16330 (ABFU31_16315) - 3734470..3735333 (+) 864 WP_011038216.1 A24 family peptidase -
  ABFU31_RS16335 (ABFU31_16320) coaE 3735347..3735970 (+) 624 WP_116921528.1 dephospho-CoA kinase -
  ABFU31_RS16340 (ABFU31_16325) - 3736164..3736419 (+) 256 Protein_3156 SymE family type I addiction module toxin -
  ABFU31_RS16345 (ABFU31_16330) - 3736518..3737852 (-) 1335 WP_012437598.1 HAMP domain-containing sensor histidine kinase -
  ABFU31_RS16350 (ABFU31_16335) - 3737845..3738522 (-) 678 WP_003490678.1 response regulator transcription factor -
  ABFU31_RS16355 (ABFU31_16340) rimK 3739172..3740047 (-) 876 WP_116921527.1 30S ribosomal protein S6--L-glutamate ligase -
  ABFU31_RS16360 (ABFU31_16345) glgX 3740541..3742670 (+) 2130 WP_116921526.1 glycogen debranching protein GlgX -
  ABFU31_RS16365 (ABFU31_16350) - 3743196..3743588 (-) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  ABFU31_RS16370 (ABFU31_16355) - 3743679..3744074 (-) 396 WP_042594933.1 hypothetical protein -
  ABFU31_RS16375 (ABFU31_16360) - 3744258..3744404 (-) 147 WP_076054137.1 hypothetical protein -
  ABFU31_RS16380 (ABFU31_16365) - 3744468..3744698 (+) 231 WP_181920809.1 hypothetical protein -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13656.88 Da        Isoelectric Point: 8.8372

>NTDB_id=1000139 ABFU31_RS16320 WP_104570927.1 3732452..3732850(-) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain bglFP 6814]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQYEVLVNEGTVPTTAAQIGLTTPTARCAV
VVSTSGITCTLNGNANKIKGKTISLNRSSADGTWSCVTGGTMDAKYKPAGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=1000139 ABFU31_RS16320 WP_104570927.1 3732452..3732850(-) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain bglFP 6814]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTAGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
ACCGATGTATCAGGACTATGTTGCCAAGTCGCAGGCGACTGCCGGTCTGGCAGAAATCACTCCTGGCAAGACGCAGTATG
AAGTGTTGGTCAACGAAGGTACTGTTCCTACGACGGCCGCCCAGATTGGCTTGACAACGCCGACTGCGCGTTGCGCTGTT
GTCGTAAGCACCTCTGGCATTACATGCACCTTGAATGGCAACGCGAACAAGATTAAAGGCAAAACCATTAGCCTTAACCG
TAGTTCCGCAGATGGTACTTGGAGCTGTGTAACTGGCGGCACCATGGACGCGAAATACAAGCCCGCCGGCTGTTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

58.394

100

0.606
  pilA Acinetobacter baumannii strain A118

51.064

100

0.545
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.667

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.47
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.447
  pilA2 Legionella pneumophila str. Paris

43.066

100

0.447
  comP Acinetobacter baylyi ADP1

39.456

100

0.439
  pilA Vibrio cholerae strain A1552

39.583

100

0.432
  pilA Vibrio cholerae C6706

39.583

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.432
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.409
  pilE Neisseria gonorrhoeae MS11

34.416

100

0.402
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

94.697

0.394
  pilA Haemophilus influenzae Rd KW20

38.346

100

0.386

Multiple sequence alignment