Detailed information NTG1000140

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: ABFU31_RS16325
• Genome accession: NZ_CP155950
• Coordinates: 3733201..3734463 (+)
• Length: 420 a.a.
• NCBI ID: WP_104570928.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. raphani strain bglFP 6814
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3721348..3745195	3733201..3734463	within	0

Gene organization within MGE regions

Location: 3721348..3745195

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU31_RS16275 (ABFU31_16260)	pilB	3722333..3724069 (-)	1737	WP_040940802.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABFU31_RS16280 (ABFU31_16265)	-	3724225..3725076 (+)	852	WP_108133901.1	glycosyltransferase family 2 protein	-
ABFU31_RS16285 (ABFU31_16270)	-	3725126..3725989 (+)	864	WP_116921534.1	glycosyltransferase	-
ABFU31_RS16290 (ABFU31_16275)	-	3725974..3727146 (+)	1173	WP_181920810.1	glycosyltransferase	-
ABFU31_RS16295 (ABFU31_16280)	-	3727140..3727982 (+)	843	WP_147308817.1	glycosyltransferase	-
ABFU31_RS16300 (ABFU31_16285)	-	3727985..3728830 (-)	846	WP_323535832.1	glycosyltransferase family 9 protein	-
ABFU31_RS16305 (ABFU31_16290)	-	3729168..3729926 (-)	759	WP_116921530.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
ABFU31_RS16310 (ABFU31_16295)	-	3730050..3731870 (-)	1821	WP_147308815.1	hypothetical protein	-
ABFU31_RS16315 (ABFU31_16300)	-	3731929..3732351 (-)	423	WP_104570926.1	pilin	-
ABFU31_RS16320 (ABFU31_16305)	pilA/pilAI	3732452..3732850 (-)	399	WP_104570927.1	pilin	Machinery gene
ABFU31_RS16325 (ABFU31_16310)	pilC	3733201..3734463 (+)	1263	WP_104570928.1	type II secretion system F family protein	Machinery gene
ABFU31_RS16330 (ABFU31_16315)	-	3734470..3735333 (+)	864	WP_011038216.1	A24 family peptidase	-
ABFU31_RS16335 (ABFU31_16320)	coaE	3735347..3735970 (+)	624	WP_116921528.1	dephospho-CoA kinase	-
ABFU31_RS16340 (ABFU31_16325)	-	3736164..3736419 (+)	256	Protein_3156	SymE family type I addiction module toxin	-
ABFU31_RS16345 (ABFU31_16330)	-	3736518..3737852 (-)	1335	WP_012437598.1	HAMP domain-containing sensor histidine kinase	-
ABFU31_RS16350 (ABFU31_16335)	-	3737845..3738522 (-)	678	WP_003490678.1	response regulator transcription factor	-
ABFU31_RS16355 (ABFU31_16340)	rimK	3739172..3740047 (-)	876	WP_116921527.1	30S ribosomal protein S6--L-glutamate ligase	-
ABFU31_RS16360 (ABFU31_16345)	glgX	3740541..3742670 (+)	2130	WP_116921526.1	glycogen debranching protein GlgX	-
ABFU31_RS16365 (ABFU31_16350)	-	3743196..3743588 (-)	393	WP_011038224.1	H-NS family nucleoid-associated regulatory protein	-
ABFU31_RS16370 (ABFU31_16355)	-	3743679..3744074 (-)	396	WP_042594933.1	hypothetical protein	-
ABFU31_RS16375 (ABFU31_16360)	-	3744258..3744404 (-)	147	WP_076054137.1	hypothetical protein	-
ABFU31_RS16380 (ABFU31_16365)	-	3744468..3744698 (+)	231	WP_181920809.1	hypothetical protein	-

Sequence

Protein

• Length: 420 a.a.
• Molecular weight: 45799.15 Da
• Isoelectric Point: 10.2311

>NTDB_id=1000140 ABFU31_RS16325 WP_104570928.1 3733201..3734463(+) (pilC) [Xanthomonas campestris pv. raphani strain bglFP 6814]
MSAVRSTTKNKPASISAEQQMSPFVWEGTDKRGVKMKGEQVARNANMLRAELRRQGITPSVVKAKPKPLFGAAGKKITPK
EIAFFSRQMATMMKSGVPIVGSLEIIGNGHKNPRMKQMVGQIRTDIEGGSSLHEAVSKHPVQFDELYRNLIKAGEGAGVL
ETVLDTIASYKENLEALKGKIKKALFYPAMVVAVALLVSSILLIWVVPQFEDVFKGFGAELPAFTQLIVNASRFMVSYWW
LMLLVIVGSLVGFIFAYKRSLAMQHGMDRLVLKVPIIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNSVYE
KAVLRMREDVSVGYPVNVSMKQVNLFPHMVIQMTAIGEEAGALDAMLFKVAEYYEQEVNNAVDALSSLIEPLIMVFIGTV
VGGMVIGMYLPIFKLASVVG

Nucleotide

• Length: 1263 bp

>NTDB_id=1000140 ABFU31_RS16325 WP_104570928.1 3733201..3734463(+) (pilC) [Xanthomonas campestris pv. raphani strain bglFP 6814]
ATGTCGGCAGTCCGCAGCACCACCAAAAACAAACCGGCGAGCATCAGCGCCGAACAGCAGATGAGTCCGTTCGTCTGGGA
GGGAACAGACAAGCGCGGTGTGAAGATGAAAGGTGAACAGGTCGCCCGCAACGCCAATATGTTGCGTGCGGAACTCCGCC
GGCAGGGCATTACGCCCAGCGTTGTTAAGGCAAAGCCTAAGCCACTGTTCGGGGCGGCAGGCAAGAAGATCACGCCGAAG
GAAATTGCGTTCTTCAGCCGTCAGATGGCCACTATGATGAAGTCGGGCGTCCCGATTGTCGGGTCGCTGGAGATCATCGG
CAATGGTCACAAAAACCCGCGCATGAAGCAGATGGTCGGGCAGATCCGTACCGACATCGAGGGCGGCTCTTCGCTGCACG
AAGCGGTAAGCAAGCATCCGGTGCAGTTCGACGAGCTGTACCGCAACCTGATCAAGGCGGGCGAAGGCGCTGGTGTGCTG
GAAACCGTCCTGGATACCATCGCGTCGTACAAGGAGAACCTGGAAGCCCTGAAGGGCAAGATCAAGAAGGCCCTGTTCTA
CCCCGCGATGGTCGTGGCAGTGGCCTTGTTGGTGAGCTCGATACTCTTGATTTGGGTCGTTCCGCAGTTCGAGGACGTGT
TCAAGGGGTTTGGCGCGGAACTGCCTGCATTTACCCAACTGATCGTTAATGCGTCCCGATTCATGGTCTCGTATTGGTGG
CTGATGCTGCTTGTGATCGTCGGCTCGCTTGTGGGCTTCATCTTTGCCTACAAGCGATCACTTGCAATGCAGCATGGCAT
GGATCGGTTGGTACTCAAGGTACCCATCATCGGCCAGATCATGCACAACAGCTCGATTGCGCGTTTTGCGCGCACTACCG
CGGTGACCTTCAAGGCAGGCGTGCCGCTGGTCGAAGCACTGGGCATCGTCGCTGGCGCCACCGGCAACTCGGTGTACGAG
AAAGCAGTATTGCGCATGCGCGAGGATGTGTCGGTGGGCTATCCGGTCAACGTGTCGATGAAACAGGTCAACCTGTTTCC
GCATATGGTGATCCAGATGACGGCGATCGGCGAGGAGGCGGGCGCATTGGACGCCATGCTGTTCAAAGTTGCCGAATACT
ACGAGCAGGAAGTGAACAATGCGGTGGACGCACTGAGCAGCCTCATCGAACCCCTGATCATGGTGTTCATTGGTACAGTA
GTCGGCGGCATGGTCATCGGCATGTACCTGCCCATCTTCAAGCTCGCTTCGGTAGTTGGATAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (85-208)

• Type II secretion system (T2SS), protein F (T2SSF) (289-411)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Acinetobacter baylyi ADP1	52.488	95.714	0.502
pilC	Legionella pneumophila strain ERS1305867	51.095	97.857	0.5
pilC	Pseudomonas stutzeri DSM 10701	52.513	94.762	0.498
pilC	Acinetobacter baumannii D1279779	50.369	96.905	0.488
pilG	Neisseria gonorrhoeae MS11	42.857	98.333	0.421
pilG	Neisseria meningitidis 44/76-A	42.271	98.571	0.417
pilC	Vibrio cholerae strain A1552	41.562	94.524	0.393
pilC	Vibrio campbellii strain DS40M4	39.9	95.476	0.381