Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 30188..30780 Replicon plasmid pJIE143
Accession JN194214
Organism Escherichia coli strain JIE143 transconjugant Tx143

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag Locus_34 Protein ID AEQ61772.1
Coordinates 30532..30780 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag Locus_33 Protein ID AEQ61773.1
Coordinates 30188..30535 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_26 26191..26517 - 327 AEQ61766.1 Pilx2 -
Locus_27 26531..27151 - 621 AEQ61767.1 Pilx1 -
Locus_28 27154..27330 - 177 AEQ61792.1 conserved hypothetical protein -
Locus_29 27509..27724 - 216 AEQ61782.1 putative Eex entry exclusion protein -
Locus_30 27721..28014 - 294 AEQ61793.1 conserved hypothetical protein -
Locus_31 28091..29320 - 1230 AEQ61771.1 TaxC DNA relaxase/nickase -
Locus_32 29324..29827 - 504 AEQ61783.1 TaxA -
Locus_33 30188..30535 - 348 AEQ61773.1 putative HicB toxin-antitoxin system protein Antitoxin
Locus_34 30532..30780 - 249 AEQ61772.1 putative HicA toxin-antitoxin system protein Toxin
Locus_35 31085..31450 - 366 AEQ61791.1 conserved hypothetical protein -
Locus_36 31526..31867 - 342 AEQ61790.1 conserved hypothetical protein -
Locus_37 31827..32186 - 360 AEQ61789.1 conserved hypothetical protein -
Locus_38 32279..32506 - 228 AEQ61788.1 conserved hypothetical protein -
Locus_39 32493..32744 - 252 AEQ61787.1 conserved hypothetical protein -
Locus_40 32741..33283 - 543 AEQ61786.1 conserved hypothetical protein -
Locus_41 33388..34266 - 879 AEQ61770.1 DnaJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaCTX-M-15 - 1..34345 34345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T20092 AEQ61772.1 JN194214:c30780-30532 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T20092 JN194214:c30780-30532 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT20092 AEQ61773.1 JN194214:c30535-30188 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT20092 JN194214:c30535-30188 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References