96772

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 26641..27233 Replicon plasmid pCFS3313-3
Accession NZ_CP026942
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag C5F74_RS25180 Protein ID WP_000520549.1
Coordinates 26985..27233 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag C5F74_RS25175 Protein ID WP_000681613.1
Coordinates 26641..26988 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS25145 22645..22917 - 273 WP_001328551.1 TrbC/VirB2 family protein -
C5F74_RS25150 22985..23605 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
C5F74_RS25155 23962..24177 - 216 WP_000760375.1 EexN family lipoprotein -
C5F74_RS25160 24174..24467 - 294 WP_000134733.1 LuxR family transcriptional regulator -
C5F74_RS25165 24544..25773 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
C5F74_RS25170 25777..26280 - 504 WP_000781813.1 hypothetical protein -
C5F74_RS25175 26641..26988 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C5F74_RS25180 26985..27233 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
C5F74_RS25185 27538..27888 - 351 WP_000854259.1 hypothetical protein -
C5F74_RS25190 27979..28278 - 300 WP_000650309.1 hypothetical protein -
C5F74_RS25195 28732..28959 - 228 WP_000801440.1 hypothetical protein -
C5F74_RS25200 28946..29197 - 252 WP_001230707.1 hypothetical protein -
C5F74_RS25205 29194..29736 - 543 WP_001083906.1 hypothetical protein -
C5F74_RS25210 29841..30356 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..31764 31764


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T96772 WP_000520549.1 NZ_CP026942:c27233-26985 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T96772 NZ_CP026942:c27233-26985 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT96772 WP_000681613.1 NZ_CP026942:c26988-26641 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT96772 NZ_CP026942:c26988-26641 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References