Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) TsxAB/-
Location 32279..32744 Replicon plasmid pJIE143
Accession JN194214
Organism Escherichia coli strain JIE143 transconjugant Tx143

Toxin (Protein)


Gene name TsxA Uniprot ID -
Locus tag - Protein ID -
Coordinates 32493..32744 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID -
Locus tag - Protein ID -
Coordinates 32279..32506 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_29 27509..27724 - 216 AEQ61782.1 putative Eex entry exclusion protein -
Locus_30 27721..28014 - 294 AEQ61793.1 conserved hypothetical protein -
Locus_31 28091..29320 - 1230 AEQ61771.1 TaxC DNA relaxase/nickase -
Locus_32 29324..29827 - 504 AEQ61783.1 TaxA -
Locus_33 30188..30535 - 348 AEQ61773.1 putative HicB toxin-antitoxin system protein -
Locus_34 30532..30780 - 249 AEQ61772.1 putative HicA toxin-antitoxin system protein -
Locus_35 31085..31450 - 366 AEQ61791.1 conserved hypothetical protein -
Locus_36 31526..31867 - 342 AEQ61790.1 conserved hypothetical protein -
Locus_37 31827..32186 - 360 AEQ61789.1 conserved hypothetical protein -
Locus_38 32279..32506 - 228 AEQ61788.1 conserved hypothetical protein Antitoxin
Locus_39 32493..32744 - 252 AEQ61787.1 conserved hypothetical protein Toxin
Locus_40 32741..33283 - 543 AEQ61786.1 conserved hypothetical protein -
Locus_41 33388..34266 - 879 AEQ61770.1 DnaJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid blaCTX-M-15 - 1..34345 34345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T10151 - JN194214:c32744-32493 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T10151 JN194214:c32744-32493 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT10151 - JN194214:c32506-32279 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT10151 JN194214:c32506-32279 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Paula Bustamante et al. (2019) The roles of HicBA and a novel toxin-antitoxin-like system, TsxAB, in the stability of IncX4 resistance plasmids in Escherichia coli. The Journal of Antimicrobial Chemotherapy 74(3):553-556. [PubMed:30535076] experimental


experimental experimental literature