168329

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7637..8130 Replicon plasmid pEc45_2
Accession NZ_CP059127
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag H0X93_RS24810 Protein ID WP_001230709.1
Coordinates 7637..7891 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag H0X93_RS24815 Protein ID WP_000151456.1
Coordinates 7888..8130 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS24785 (H0X93_24680) 3119..3646 - 528 WP_000577043.1 DUF2726 domain-containing protein -
H0X93_RS24790 (H0X93_24685) 3781..4185 - 405 WP_012421203.1 hypothetical protein -
H0X93_RS24795 (H0X93_24690) 4198..5064 - 867 WP_001353743.1 replication initiation protein -
H0X93_RS24800 (H0X93_24695) 6479..6994 + 516 WP_001025393.1 J domain-containing protein -
H0X93_RS24805 (H0X93_24700) 7098..7640 + 543 WP_001083904.1 hypothetical protein -
H0X93_RS24810 (H0X93_24705) 7637..7891 + 255 WP_001230709.1 hypothetical protein Toxin
H0X93_RS24815 (H0X93_24710) 7888..8130 + 243 WP_000151456.1 hypothetical protein Antitoxin
H0X93_RS24820 (H0X93_24715) 8403..8585 + 183 WP_071607788.1 hypothetical protein -
H0X93_RS24825 (H0X93_24720) 8701..8988 + 288 WP_000673430.1 hypothetical protein -
H0X93_RS24830 (H0X93_24725) 9040..9414 + 375 WP_000844302.1 tail-associated lysozyme -
H0X93_RS24835 (H0X93_24730) 9429..9722 + 294 WP_000954637.1 hypothetical protein -
H0X93_RS24840 (H0X93_24735) 10026..10274 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
H0X93_RS24845 (H0X93_24740) 10271..10618 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
H0X93_RS24850 (H0X93_24745) 10979..11482 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
H0X93_RS24855 (H0X93_24750) 11486..12715 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
H0X93_RS24860 (H0X93_24755) 12792..13085 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..32524 32524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type I toxin-antitoxin system, toxin Ldr

(1-35)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T168329 WP_001230709.1 NZ_CP059127:7637-7891 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T168329 NZ_CP075722:2607993-2608100 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT168329 WP_000151456.1 NZ_CP059127:7888-8130 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT168329 NZ_CP075722:c2607946-2607880 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References