77226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 12569..13161 Replicon plasmid unitig_4
Accession NZ_CP021735
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag AM361_RS25965 Protein ID WP_000520549.1
Coordinates 12913..13161 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag AM361_RS25960 Protein ID WP_000681613.1
Coordinates 12569..12916 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS25925 8572..8844 - 273 WP_001328551.1 TrbC/VirB2 family protein -
AM361_RS25930 8912..9532 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
AM361_RS25935 9890..10105 - 216 WP_000760375.1 EexN family lipoprotein -
AM361_RS25940 10102..10395 - 294 WP_000134733.1 LuxR family transcriptional regulator -
AM361_RS25945 10472..11701 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
AM361_RS25950 11705..12208 - 504 WP_000781813.1 hypothetical protein -
AM361_RS25960 12569..12916 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AM361_RS25965 12913..13161 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
AM361_RS25970 13466..13816 - 351 WP_000854259.1 hypothetical protein -
AM361_RS25975 13907..14206 - 300 WP_000650309.1 hypothetical protein -
AM361_RS25985 14660..14887 - 228 WP_000801440.1 hypothetical protein -
AM361_RS25990 14874..15125 - 252 WP_001230707.1 hypothetical protein -
AM361_RS25995 15122..15664 - 543 WP_001083906.1 hypothetical protein -
AM361_RS26500 15769..16284 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..31765 31765


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T77226 WP_000520549.1 NZ_CP021735:c13161-12913 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T77226 NZ_CP021735:c13161-12913 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT77226 WP_000681613.1 NZ_CP021735:c12916-12569 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT77226 NZ_CP021735:c12916-12569 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References