77227

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 14660..15125 Replicon plasmid unitig_4
Accession NZ_CP021735
Organism Escherichia coli strain AR_0114

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag AM361_RS25990 Protein ID WP_001230707.1
Coordinates 14874..15125 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag AM361_RS25985 Protein ID WP_000801440.1
Coordinates 14660..14887 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM361_RS25935 9890..10105 - 216 WP_000760375.1 EexN family lipoprotein -
AM361_RS25940 10102..10395 - 294 WP_000134733.1 LuxR family transcriptional regulator -
AM361_RS25945 10472..11701 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
AM361_RS25950 11705..12208 - 504 WP_000781813.1 hypothetical protein -
AM361_RS25960 12569..12916 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
AM361_RS25965 12913..13161 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
AM361_RS25970 13466..13816 - 351 WP_000854259.1 hypothetical protein -
AM361_RS25975 13907..14206 - 300 WP_000650309.1 hypothetical protein -
AM361_RS25985 14660..14887 - 228 WP_000801440.1 hypothetical protein Antitoxin
AM361_RS25990 14874..15125 - 252 WP_001230707.1 hypothetical protein Toxin
AM361_RS25995 15122..15664 - 543 WP_001083906.1 hypothetical protein -
AM361_RS26500 15769..16284 - 516 WP_001025395.1 J domain-containing protein -
AM361_RS26005 17699..18565 + 867 WP_001353743.1 replication initiation protein -
AM361_RS26010 18578..18982 + 405 WP_012421203.1 hypothetical protein -
AM361_RS26015 19117..19644 + 528 WP_000577043.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..31765 31765


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-73)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T77227 WP_001230707.1 NZ_CP021735:c15125-14874 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T77227 NZ_CP021735:c15125-14874 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT77227 WP_000801440.1 NZ_CP021735:c14887-14660 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT77227 NZ_CP021735:c14887-14660 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References