168330

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 10026..10618 Replicon plasmid pEc45_2
Accession NZ_CP059127
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag H0X93_RS24840 Protein ID WP_000520549.1
Coordinates 10026..10274 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag H0X93_RS24845 Protein ID WP_000681613.1
Coordinates 10271..10618 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS24800 (H0X93_24695) 6479..6994 + 516 WP_001025393.1 J domain-containing protein -
H0X93_RS24805 (H0X93_24700) 7098..7640 + 543 WP_001083904.1 hypothetical protein -
H0X93_RS24810 (H0X93_24705) 7637..7891 + 255 WP_001230709.1 hypothetical protein -
H0X93_RS24815 (H0X93_24710) 7888..8130 + 243 WP_000151456.1 hypothetical protein -
H0X93_RS24820 (H0X93_24715) 8403..8585 + 183 WP_071607788.1 hypothetical protein -
H0X93_RS24825 (H0X93_24720) 8701..8988 + 288 WP_000673430.1 hypothetical protein -
H0X93_RS24830 (H0X93_24725) 9040..9414 + 375 WP_000844302.1 tail-associated lysozyme -
H0X93_RS24835 (H0X93_24730) 9429..9722 + 294 WP_000954637.1 hypothetical protein -
H0X93_RS24840 (H0X93_24735) 10026..10274 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
H0X93_RS24845 (H0X93_24740) 10271..10618 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H0X93_RS24850 (H0X93_24745) 10979..11482 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
H0X93_RS24855 (H0X93_24750) 11486..12715 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
H0X93_RS24860 (H0X93_24755) 12792..13085 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
H0X93_RS24865 (H0X93_24760) 13082..13297 + 216 WP_000760375.1 EexN family lipoprotein -
H0X93_RS24870 (H0X93_24765) 13654..14274 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
H0X93_RS24875 (H0X93_24770) 14288..14614 + 327 WP_000008647.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..32524 32524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T168330 WP_000520549.1 NZ_CP059127:10026-10274 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T168330 NZ_CP075722:2608528-2608635 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT168330 WP_000681613.1 NZ_CP059127:10271-10618 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT168330 NZ_CP075722:c2608481-2608415 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References