215302

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 28691..29283 Replicon plasmid unnamed3
Accession NZ_CP082830
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag K8B73_RS26235 Protein ID WP_000520549.1
Coordinates 28691..28939 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag K8B73_RS26240 Protein ID WP_000681613.1
Coordinates 28936..29283 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS26205 (K8B73_26205) 25566..26081 + 516 WP_001025395.1 J domain-containing protein -
K8B73_RS26210 (K8B73_26210) 26186..26728 + 543 WP_001083906.1 hypothetical protein -
K8B73_RS26215 (K8B73_26215) 26725..26976 + 252 WP_001230707.1 hypothetical protein -
K8B73_RS26220 (K8B73_26220) 26963..27190 + 228 WP_000801440.1 hypothetical protein -
K8B73_RS26225 (K8B73_26225) 27646..27945 + 300 WP_000650309.1 hypothetical protein -
K8B73_RS26230 (K8B73_26230) 28036..28386 + 351 WP_000854259.1 hypothetical protein -
K8B73_RS26235 (K8B73_26235) 28691..28939 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
K8B73_RS26240 (K8B73_26240) 28936..29283 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K8B73_RS26245 (K8B73_26245) 29644..30147 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
K8B73_RS26250 (K8B73_26250) 30151..31380 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
K8B73_RS26255 (K8B73_26255) 31457..31750 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
K8B73_RS26260 (K8B73_26260) 31747..31962 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..32040 32040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T215302 WP_000520549.1 NZ_CP082830:28691-28939 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T215302 NZ_CP110621:1874116-1874442 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAATG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT215302 WP_000681613.1 NZ_CP082830:28936-29283 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT215302 NZ_CP110621:1874529-1874855 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTTGTCTATTGATCAGCATAACCTGTTAGAAAAAAT
CGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References