149102

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 27986..28578 Replicon plasmid p010_C
Accession NZ_CP048333
Organism Escherichia coli strain 10

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag GZS13_RS25010 Protein ID WP_000520549.1
Coordinates 28330..28578 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag GZS13_RS25005 Protein ID WP_000681613.1
Coordinates 27986..28333 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS13_RS24975 23988..24260 - 273 WP_001328551.1 TrbC/VirB2 family protein -
GZS13_RS24980 24328..24948 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
GZS13_RS24985 25307..25522 - 216 WP_000760375.1 EexN family lipoprotein -
GZS13_RS24990 25519..25812 - 294 WP_000134733.1 LuxR family transcriptional regulator -
GZS13_RS24995 25889..27118 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
GZS13_RS25000 27122..27625 - 504 WP_000781813.1 hypothetical protein -
GZS13_RS25005 27986..28333 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GZS13_RS25010 28330..28578 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
GZS13_RS25015 28883..29233 - 351 WP_000854259.1 hypothetical protein -
GZS13_RS25020 29324..29623 - 300 WP_000650309.1 hypothetical protein -
GZS13_RS25025 30077..30304 - 228 WP_000801440.1 hypothetical protein -
GZS13_RS25030 30291..30542 - 252 WP_001230707.1 hypothetical protein -
GZS13_RS25035 30539..31081 - 543 WP_001083906.1 hypothetical protein -
GZS13_RS25040 31186..31701 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..32102 32102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T149102 WP_000520549.1 NZ_CP048333:c28578-28330 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T149102 NZ_CP063928:c28172-27690 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT149102 WP_000681613.1 NZ_CP048333:c28333-27986 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT149102 NZ_CP063928:c28426-28160 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References