215301

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 26725..27190 Replicon plasmid unnamed3
Accession NZ_CP082830
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag K8B73_RS26215 Protein ID WP_001230707.1
Coordinates 26725..26976 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag K8B73_RS26220 Protein ID WP_000801440.1
Coordinates 26963..27190 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS26190 (K8B73_26190) 22268..22711 - 444 WP_223107168.1 hypothetical protein -
K8B73_RS26195 (K8B73_26195) 22846..23250 - 405 WP_012421203.1 hypothetical protein -
K8B73_RS26200 (K8B73_26200) 23263..24129 - 867 WP_001353743.1 replication initiation protein -
K8B73_RS26205 (K8B73_26205) 25566..26081 + 516 WP_001025395.1 J domain-containing protein -
K8B73_RS26210 (K8B73_26210) 26186..26728 + 543 WP_001083906.1 hypothetical protein -
K8B73_RS26215 (K8B73_26215) 26725..26976 + 252 WP_001230707.1 hypothetical protein Toxin
K8B73_RS26220 (K8B73_26220) 26963..27190 + 228 WP_000801440.1 hypothetical protein Antitoxin
K8B73_RS26225 (K8B73_26225) 27646..27945 + 300 WP_000650309.1 hypothetical protein -
K8B73_RS26230 (K8B73_26230) 28036..28386 + 351 WP_000854259.1 hypothetical protein -
K8B73_RS26235 (K8B73_26235) 28691..28939 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
K8B73_RS26240 (K8B73_26240) 28936..29283 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
K8B73_RS26245 (K8B73_26245) 29644..30147 + 504 WP_000781813.1 relaxosome-coupling protein TaxA -
K8B73_RS26250 (K8B73_26250) 30151..31380 + 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
K8B73_RS26255 (K8B73_26255) 31457..31750 + 294 WP_000134733.1 sigma factor-like helix-turn-helix DNA-binding protein -
K8B73_RS26260 (K8B73_26260) 31747..31962 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Non-Mobilizable plasmid - - 1..32040 32040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T215301 WP_001230707.1 NZ_CP082830:26725-26976 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T215301 NZ_CP110621:c884372-884088 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT215301 WP_000801440.1 NZ_CP082830:26963-27190 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT215301 NZ_CP110621:c884091-883798 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References