TAfinder detailed results

Overview

Job ID wrVbGpMxFf
Sequence name NZ_CP026085.1 Escherichia coli strain DH5alpha chromosome, complete genome
Predicted type II

Toxin (Protein)


Predicted family vapC (T439) Predicted domain PRK13725 (VapC)
Locus tag orf1244 Length 132 a.a.
Coordinates 1329386..1329784 (+)

Antitoxin (Protein)


Predicted family vapB (AT439) Predicted domain COG4456 (VagC)
Locus tag orf1243 Length 88 a.a.
Coordinates 1329120..1329386 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
orf1237 1324961..1325431 - 471 ORF1237 Predicted ORF by PRODIGAL Version 1.20 -
orf1238 1325431..1326003 - 573 ORF1238 Predicted ORF by PRODIGAL Version 1.20 -
orf1239 1326149..1327027 + 879 ORF1239 Predicted ORF by PRODIGAL Version 1.20 -
orf1240 1327044..1328078 + 1035 ORF1240 Predicted ORF by PRODIGAL Version 1.20 -
orf1241 1327989..1328219 - 231 ORF1241 Predicted ORF by PRODIGAL Version 1.20 -
orf1242 1328291..1329004 + 714 ORF1242 Predicted ORF by PRODIGAL Version 1.20 -
orf1243 1329120..1329386 + 267 ORF1243 Predicted ORF by PRODIGAL Version 1.20 Antitoxin
orf1244 1329386..1329784 + 399 ORF1244 Predicted ORF by PRODIGAL Version 1.20 Toxin
orf1245 1329931..1330794 + 864 ORF1245 Predicted ORF by PRODIGAL Version 1.20 -
orf1246 1330809..1332824 + 2016 ORF1246 Predicted ORF by PRODIGAL Version 1.20 -
orf1247 1332898..1333596 + 699 ORF1247 Predicted ORF by PRODIGAL Version 1.20 -
orf1248 1333677..1333877 - 201 ORF1248 Predicted ORF by PRODIGAL Version 1.20 -

Domains

The domains were predicted by HMMER and were sorted by score.

Toxin

PRK13725 (VapC) (E-value: 3.5e-79, Score:261.4)

(1-131)

cd09881 (VapC) (E-value: 2.6e-18, Score:64.3)

(2-121)

COG1487 (VapC) (E-value: 2.4e-09, Score:35.4)

(6-130)

cd09875 (VapC) (E-value: 9.3e-07, Score:27.2)

(5-122)

PF01850 (PIN) (E-value: 0.00072, Score:17.7)

(4-112)

cd09886 (NGN_SP) (E-value: 0.0014, Score:16.7)

(3-107)

cd09861 (VapC) (E-value: 0.0014, Score:16.7)

(3-107)

cd09872 (PIN_Sll0205-like) (E-value: 0.013, Score:13.5)

(2-114)

COG5573 (PIN) (E-value: 0.022, Score:12.7)

(5-94)

Antitoxin

COG4456 (VagC) (E-value: 1.8e-26, Score:90)

(4-79)

PF04014 (MazE) (E-value: 3.4e-08, Score:31.8)

(4-77)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>ORF1244 TA_12 Toxin
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVI
EGFVSRLEVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREF
ERVAGIRLEDWS

Download         Length: 399 bp

>ORF1244 TA_12 Toxin
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTA
CATGTCAGAGAGCGGTTTAACCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTG
ATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAGAACGCAATTTGGCGGTGATT
GAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATG
ATAGCGGGACATGCTCGCAGTCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTT
GAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.32 Da        Isoelectric Point: 4.7962

>ORF1243 TA_12 Antitoxin
MYIHDVYPKKGGAMETTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESW
DSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 267 bp

>ORF1243 TA_12 Antitoxin
ATGTATATACATGACGTATATCCCAAAAAAGGGGGGGCTATGGAAACTACTGTATTTTTA
AGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAAG
AAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGG
GACAGTTGGTTTGATGGCGAAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCC
GCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.