Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QMG88_RS16395 Genome accession   NZ_AP027052
Coordinates   3582530..3583636 (-) Length   368 a.a.
NCBI ID   WP_004398310.1    Uniprot ID   A0AAV2VQ18
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3577530..3588636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG88_RS16365 (TUMSATVNIG3_31970) - 3578291..3578791 (+) 501 WP_281827654.1 SprT family zinc-dependent metalloprotease -
  QMG88_RS16370 (TUMSATVNIG3_31980) - 3578879..3579580 (+) 702 WP_022549510.1 endonuclease -
  QMG88_RS16375 (TUMSATVNIG3_31990) rsmE 3579686..3580417 (+) 732 WP_281827655.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QMG88_RS16380 (TUMSATVNIG3_32000) gshB 3580432..3581379 (+) 948 WP_281827656.1 glutathione synthase -
  QMG88_RS16385 (TUMSATVNIG3_32010) - 3581406..3581969 (+) 564 WP_004409298.1 YqgE/AlgH family protein -
  QMG88_RS16390 (TUMSATVNIG3_32020) ruvX 3582022..3582447 (+) 426 WP_022549508.1 Holliday junction resolvase RuvX -
  QMG88_RS16395 (TUMSATVNIG3_32030) pilU 3582530..3583636 (-) 1107 WP_004398310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMG88_RS16400 (TUMSATVNIG3_32040) pilT 3583645..3584682 (-) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG88_RS16405 (TUMSATVNIG3_32050) - 3584706..3585416 (+) 711 WP_004398312.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMG88_RS16410 (TUMSATVNIG3_32060) proC 3585478..3586296 (+) 819 WP_281827657.1 pyrroline-5-carboxylate reductase -
  QMG88_RS16415 (TUMSATVNIG3_32070) - 3586326..3586883 (+) 558 WP_004398314.1 YggT family protein -
  QMG88_RS16420 (TUMSATVNIG3_32080) yggU 3586883..3587173 (+) 291 WP_022549505.1 DUF167 family protein YggU -
  QMG88_RS16425 (TUMSATVNIG3_32090) - 3587197..3587628 (+) 432 WP_281827658.1 DUF4426 domain-containing protein -
  QMG88_RS16430 (TUMSATVNIG3_32100) - 3587695..3588294 (+) 600 WP_022601627.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41280.30 Da        Isoelectric Point: 5.6292

>NTDB_id=99386 QMG88_RS16395 WP_004398310.1 3582530..3583636(-) (pilU) [Vibrio nigripulchritudo strain TUMSAT-V.nig3]
MELNQVLSEMVSLKASDIYITVGAPVLLRVHGELQPLGEPLTEQSAFHLLDTMMDEERRAEFRKERESNFAIVRDVGRFR
VSAFFQRELPGAVLRRIETQIPTFEELKLPDVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNQCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGQGRHGVFEILLNTPRVSDLIRRGELHELKATMAKSNEAGMKTFDQCLY
ELLSADKITEQDAMHSADSANDLRLMMKTKQGGDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=99386 QMG88_RS16395 WP_004398310.1 3582530..3583636(-) (pilU) [Vibrio nigripulchritudo strain TUMSAT-V.nig3]
ATGGAACTGAATCAAGTTTTATCAGAAATGGTGTCTCTGAAGGCATCAGACATCTACATCACGGTAGGTGCGCCAGTCCT
GTTACGTGTTCACGGAGAACTGCAACCACTGGGTGAACCACTGACTGAGCAGTCTGCCTTTCATCTACTCGATACAATGA
TGGATGAAGAGCGTCGCGCGGAATTTCGAAAAGAAAGAGAGTCCAACTTTGCCATCGTTCGAGATGTTGGACGTTTTCGT
GTGAGTGCGTTCTTTCAGCGTGAACTGCCTGGTGCGGTATTGCGCCGAATCGAGACGCAAATCCCTACCTTCGAAGAGCT
CAAATTACCGGATGTTCTGCAGGATCTTGCCATCGCTAAGCGAGGTTTAGTGCTGGTGGTTGGTGCGACTGGCTCAGGTA
AGTCCACCACCATGGCTGCAATGACGGGCTTTCGAAATCAGAATCGCACAGGTCATATCCTGACAGTGGAAGACCCGATT
GAATTTGTTCACGAGCATAACCAGTGCATCATCACCCAGCGTGAAGTGGGTTTGGATACCGAGAGCTATGAAGTCGCACT
GAAGAATTCACTTCGACAAGCACCAGACATGATTCTGATTGGTGAAATTCGCTCCCGAGAAACCATGGAATATGCCATGA
CTTTTGCGGAAACCGGTCACCTCTGCATGGCAACACTGCACGCTAACAACGCAAACCAGGCATTGGAACGTATTCTTCAC
CTAGTGCCAAAAGAGCAGAAAGAGCAGTTCCTGTTTGATTTATCCATGAACTTGCGTGGTGTGATAGCGCAGCAACTGAT
TCGCGATAAGAGCGGGCAAGGGCGGCATGGTGTATTTGAGATTCTTCTGAACACCCCAAGGGTATCCGACCTGATTCGTC
GTGGTGAGCTGCACGAGCTGAAAGCAACGATGGCGAAATCCAACGAGGCGGGGATGAAAACCTTCGACCAGTGTCTGTAT
GAGCTTCTGTCGGCAGACAAGATCACCGAGCAAGATGCGATGCACAGCGCTGACTCTGCGAATGATTTGAGGTTGATGAT
GAAAACCAAGCAAGGCGGAGATTTCTCTGGCGGCGCGCTTGATGGTGTGAAAATAGACATGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

80.381

99.728

0.802
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter nosocomialis M2

41.471

92.391

0.383
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

41.176

92.391

0.38
  pilT Acinetobacter baumannii strain A118

41.176

92.391

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Legionella pneumophila strain Lp02

40.118

92.12

0.37
  pilT Legionella pneumophila strain ERS1305867

40.118

92.12

0.37
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment