Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QMG95_RS16710 Genome accession   NZ_AP027045
Coordinates   3625524..3626630 (-) Length   368 a.a.
NCBI ID   WP_004398310.1    Uniprot ID   A0AAV2VQ18
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620524..3631630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG95_RS16680 (TUMSATVNIG1_32670) - 3621286..3621786 (+) 501 WP_200766202.1 SprT family zinc-dependent metalloprotease -
  QMG95_RS16685 (TUMSATVNIG1_32680) - 3621874..3622575 (+) 702 WP_200766203.1 endonuclease -
  QMG95_RS16690 (TUMSATVNIG1_32690) rsmE 3622681..3623412 (+) 732 WP_022593808.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QMG95_RS16695 (TUMSATVNIG1_32700) gshB 3623427..3624374 (+) 948 WP_022549509.1 glutathione synthase -
  QMG95_RS16700 (TUMSATVNIG1_32710) - 3624401..3624964 (+) 564 WP_004409298.1 YqgE/AlgH family protein -
  QMG95_RS16705 (TUMSATVNIG1_32720) ruvX 3625017..3625442 (+) 426 WP_022591347.1 Holliday junction resolvase RuvX -
  QMG95_RS16710 (TUMSATVNIG1_32730) pilU 3625524..3626630 (-) 1107 WP_004398310.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMG95_RS16715 (TUMSATVNIG1_32740) pilT 3626639..3627676 (-) 1038 WP_004398311.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG95_RS16720 (TUMSATVNIG1_32750) - 3627700..3628410 (+) 711 WP_004398312.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMG95_RS16725 (TUMSATVNIG1_32760) proC 3628471..3629289 (+) 819 WP_004398313.1 pyrroline-5-carboxylate reductase -
  QMG95_RS16730 (TUMSATVNIG1_32770) - 3629319..3629876 (+) 558 WP_004398314.1 YggT family protein -
  QMG95_RS16735 (TUMSATVNIG1_32780) yggU 3629876..3630166 (+) 291 WP_004398315.1 DUF167 family protein YggU -
  QMG95_RS16740 (TUMSATVNIG1_32790) - 3630190..3630621 (+) 432 WP_200766204.1 DUF4426 domain-containing protein -
  QMG95_RS16745 (TUMSATVNIG1_32800) - 3630688..3631287 (+) 600 WP_022549504.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41280.30 Da        Isoelectric Point: 5.6292

>NTDB_id=99296 QMG95_RS16710 WP_004398310.1 3625524..3626630(-) (pilU) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
MELNQVLSEMVSLKASDIYITVGAPVLLRVHGELQPLGEPLTEQSAFHLLDTMMDEERRAEFRKERESNFAIVRDVGRFR
VSAFFQRELPGAVLRRIETQIPTFEELKLPDVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNQCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGQGRHGVFEILLNTPRVSDLIRRGELHELKATMAKSNEAGMKTFDQCLY
ELLSADKITEQDAMHSADSANDLRLMMKTKQGGDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=99296 QMG95_RS16710 WP_004398310.1 3625524..3626630(-) (pilU) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
ATGGAACTGAATCAAGTTTTATCAGAAATGGTGTCTCTGAAGGCATCAGATATCTACATCACAGTGGGTGCACCTGTTTT
GTTGCGTGTTCACGGAGAACTGCAACCATTGGGTGAACCACTGACCGAGCAGTCTGCCTTTCATCTGCTCGATACTATGA
TGGATGAAGAGCGTCGCGCTGAGTTTCGTAAAGAAAGAGAGTCCAACTTTGCCATCGTTCGAGATGTTGGACGTTTTCGT
GTGAGTGCGTTCTTCCAGCGTGAACTACCCGGTGCGGTATTGCGCCGAATAGAGACGCAAATCCCTACCTTCGAAGAGCT
CAAATTACCGGATGTTCTGCAAGATCTTGCCATCGCTAAGCGAGGCTTAGTACTGGTGGTTGGTGCGACTGGCTCAGGTA
AGTCTACCACCATGGCTGCGATGACGGGCTTTCGAAATCAGAATCGCACAGGTCATATCCTGACGGTGGAAGACCCGATT
GAATTTGTTCACGAGCATAACCAGTGCATCATCACCCAGCGTGAAGTGGGTTTGGATACCGAGAGCTATGAAGTCGCACT
GAAGAATTCACTTCGACAAGCACCAGACATGATTCTGATTGGTGAAATTCGCTCCCGAGAAACCATGGAATATGCCATGA
CCTTTGCGGAAACTGGTCACCTCTGCATGGCAACACTGCACGCTAACAACGCAAACCAGGCATTGGAACGTATTCTTCAC
CTGGTGCCAAAAGAGCAGAAAGAGCAGTTCCTGTTTGATTTATCCATGAACTTACGTGGCGTGATAGCGCAGCAACTGAT
TCGCGATAAGAGCGGGCAAGGGCGGCATGGTGTATTTGAGATTCTTCTGAATACCCCAAGGGTGTCCGACCTGATTCGTC
GTGGTGAGCTGCACGAGCTGAAAGCTACGATGGCGAAATCCAACGAGGCGGGGATGAAAACCTTCGACCAGTGTTTGTAT
GAGCTTCTGTCGGCAGACAAGATCACCGAGCAAGATGCAATGCACAGCGCTGACTCTGCGAATGATTTGAGATTGATGAT
GAAAACCAAGCAAGGCGGGGATTTCTCTGGCGGCGCGCTTGATGGAGTGAAAATAGACATGAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

80.381

99.728

0.802
  pilU Pseudomonas stutzeri DSM 10701

57.714

95.109

0.549
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter nosocomialis M2

41.471

92.391

0.383
  pilT Acinetobacter baylyi ADP1

41.349

92.663

0.383
  pilT Acinetobacter baumannii D1279779

41.176

92.391

0.38
  pilT Acinetobacter baumannii strain A118

41.176

92.391

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Legionella pneumophila strain Lp02

40.118

92.12

0.37
  pilT Legionella pneumophila strain ERS1305867

40.118

92.12

0.37
  pilT Pseudomonas aeruginosa PAK

39.823

92.12

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment