Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAIA70_RS13945 Genome accession   NZ_CP154264
Coordinates   3026657..3027763 (-) Length   368 a.a.
NCBI ID   WP_343291012.1    Uniprot ID   -
Organism   Vibrio harveyi strain 45T2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3021657..3032763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA70_RS13915 (AAIA70_13915) - 3022242..3022739 (+) 498 WP_239912668.1 SprT family zinc-dependent metalloprotease -
  AAIA70_RS13920 (AAIA70_13920) endA 3022891..3023586 (+) 696 WP_005449743.1 deoxyribonuclease I -
  AAIA70_RS13925 (AAIA70_13925) rsmE 3023733..3024464 (+) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAIA70_RS13930 (AAIA70_13930) gshB 3024479..3025429 (+) 951 WP_005449741.1 glutathione synthase -
  AAIA70_RS13935 (AAIA70_13935) - 3025559..3026122 (+) 564 WP_005449740.1 YqgE/AlgH family protein -
  AAIA70_RS13940 (AAIA70_13940) ruvX 3026172..3026597 (+) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  AAIA70_RS13945 (AAIA70_13945) pilU 3026657..3027763 (-) 1107 WP_343291012.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAIA70_RS13950 (AAIA70_13950) pilT 3027795..3028835 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  AAIA70_RS13955 (AAIA70_13955) - 3028864..3029574 (+) 711 WP_009697582.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAIA70_RS13960 (AAIA70_13960) proC 3029706..3030524 (+) 819 WP_038899445.1 pyrroline-5-carboxylate reductase -
  AAIA70_RS13965 (AAIA70_13965) - 3030578..3031135 (+) 558 WP_005425806.1 YggT family protein -
  AAIA70_RS13970 (AAIA70_13970) yggU 3031135..3031425 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  AAIA70_RS13975 (AAIA70_13975) - 3031486..3031917 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  AAIA70_RS13980 (AAIA70_13980) - 3032023..3032625 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41263.37 Da        Isoelectric Point: 6.7730

>NTDB_id=992798 AAIA70_RS13945 WP_343291012.1 3026657..3027763(-) (pilU) [Vibrio harveyi strain 45T2]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPERRQEFRKSRESNFAVVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=992798 AAIA70_RS13945 WP_343291012.1 3026657..3027763(-) (pilU) [Vibrio harveyi strain 45T2]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCAA
TGGACCCGGAGCGACGACAAGAGTTTCGCAAAAGCCGTGAGTCGAACTTTGCCGTTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAATTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37

Multiple sequence alignment