Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   SP4011_RS01255 Genome accession   NZ_AP026968
Coordinates   224103..225044 (+) Length   313 a.a.
NCBI ID   WP_338619537.1    Uniprot ID   -
Organism   Streptococcus parapneumoniae strain SP4011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219103..230044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP4011_RS01235 (SP4011_02200) - 219167..220984 (+) 1818 WP_338619532.1 acyltransferase family protein -
  SP4011_RS01240 (SP4011_02210) nagA 221137..222288 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  SP4011_RS01245 (SP4011_02220) - 222451..223509 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  SP4011_RS01250 (SP4011_02230) - 223660..224025 (+) 366 WP_338619535.1 DUF1033 family protein -
  SP4011_RS01255 (SP4011_02240) comGA/cglA/cilD 224103..225044 (+) 942 WP_338619537.1 competence type IV pilus ATPase ComGA Machinery gene
  SP4011_RS01260 (SP4011_02250) comGB/cglB 224992..226008 (+) 1017 WP_338620380.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP4011_RS01265 (SP4011_02260) comGC/cglC 226010..226333 (+) 324 WP_338619538.1 competence type IV pilus major pilin ComGC Machinery gene
  SP4011_RS01270 (SP4011_02270) comGD/cglD 226296..226730 (+) 435 WP_044012294.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP4011_RS01275 (SP4011_02280) comGE/cglE 226693..226995 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP4011_RS01280 (SP4011_02290) comGF/cglF 226958..227419 (+) 462 WP_000250511.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP4011_RS01285 (SP4011_02300) comGG/cglG 227397..227810 (+) 414 WP_000265635.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP4011_RS01290 (SP4011_02310) - 228272..229114 (-) 843 WP_000840058.1 helix-turn-helix domain-containing protein -
  SP4011_RS01295 (SP4011_02320) - 229311..229787 (-) 477 WP_000777684.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35599.61 Da        Isoelectric Point: 5.6405

>NTDB_id=99077 SP4011_RS01255 WP_338619537.1 224103..225044(+) (comGA/cglA/cilD) [Streptococcus parapneumoniae strain SP4011]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRIGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGQESLVIRLLHDEEQDLHFWFQDIGELCKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=99077 SP4011_RS01255 WP_338619537.1 224103..225044(+) (comGA/cglA/cilD) [Streptococcus parapneumoniae strain SP4011]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCACAAGACATTTATTTTGTCCCCAAGTT
AGACGCATATGAGCTTCATATGAGGATAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAATTTGCGGCCG
TCATCAGTCACTTCAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGTGATTATGAA
TATGACCAGAAGATGGCGTCTCTACGTCTATCTACTGTAGGCGATTATAGAGGGCAGGAGAGTTTAGTCATTCGCTTGTT
GCACGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGGAGAACTGTGTAAGCAGTATAGGCAACGTGGTCTCT
ATCTCTTTGCTGGTCCAGTCGGCAGTGGCAAGACGACCTTGATGCACGAATTAGCCAAGTCCCTCTTTAAAGGACAGCAA
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGAGGACATGCTCCAGTTGCAGCTAAATGAAGCGATTGGGCTTAC
CTATGAAAATCTGATTAAGCTTTCCTTACGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGAGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCTTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGTGTCTGCTACCAGAGATTGATCGG
GGGAGGAGGAATTGTTGATTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.444

100

0.974
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

94.888

100

0.949
  comGA/cglA/cilD Streptococcus pneumoniae D39

94.888

100

0.949
  comGA/cglA/cilD Streptococcus pneumoniae R6

94.888

100

0.949
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

94.888

100

0.949
  comYA Streptococcus gordonii str. Challis substr. CH1

79.677

99.042

0.789
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

55.591

100

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

42.279

86.901

0.367

Multiple sequence alignment