Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   SP4011_RS01280 Genome accession   NZ_AP026968
Coordinates   226958..227419 (+) Length   153 a.a.
NCBI ID   WP_000250511.1    Uniprot ID   -
Organism   Streptococcus parapneumoniae strain SP4011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 228272..236321 226958..227419 flank 853


Gene organization within MGE regions


Location: 226958..236321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP4011_RS01280 (SP4011_02290) comGF/cglF 226958..227419 (+) 462 WP_000250511.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP4011_RS01285 (SP4011_02300) comGG/cglG 227397..227810 (+) 414 WP_000265635.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP4011_RS01290 (SP4011_02310) - 228272..229114 (-) 843 WP_000840058.1 helix-turn-helix domain-containing protein -
  SP4011_RS01295 (SP4011_02320) - 229311..229787 (-) 477 WP_000777684.1 GNAT family N-acetyltransferase -
  SP4011_RS01300 (SP4011_02330) - 229900..231102 (-) 1203 WP_000855226.1 ATP-grasp domain-containing protein -
  SP4011_RS01305 (SP4011_02340) - 231104..232003 (-) 900 WP_000771264.1 hypothetical protein -
  SP4011_RS01310 (SP4011_02350) - 232004..233065 (-) 1062 WP_000604329.1 ATP-grasp domain-containing protein -
  SP4011_RS01315 (SP4011_02360) - 233209..234192 (-) 984 WP_000864435.1 hypothetical protein -
  SP4011_RS01320 (SP4011_02370) - 234167..235186 (-) 1020 WP_000612003.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17868.55 Da        Isoelectric Point: 9.3016

>NTDB_id=99082 SP4011_RS01280 WP_000250511.1 226958..227419(+) (comGF/cglF) [Streptococcus parapneumoniae strain SP4011]
MVQNSCWLSKSHKVKAFTLLESLIALIVISGSLLLFQAMSQLLISDVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKEIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQVVRFRFQFKKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=99082 SP4011_RS01280 WP_000250511.1 226958..227419(+) (comGF/cglF) [Streptococcus parapneumoniae strain SP4011]
ATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCTTGATTGCCCTCAT
TGTCATCAGTGGAAGCTTACTTCTCTTTCAAGCCATGAGTCAGCTCCTCATTTCAGATGTTCGCTATCAGCAGCAAAGCG
AGCAAAAAGAGTGGCTCTTGTTTGTGGACCAGTTGGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTCTATATGAAGCAGGATGGCAAGGAAATTGCGATAGGCAAGTCTAAATCAGATGATTTTCGGAAAACCGATGCCAG
TGGACGGGGTTATCAGCCGATGGTTTATGGCCTCAAATCCGCACAGATTACAGAGGACAATCAAGTGGTTCGCTTTCGTT
TCCAGTTCAAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(47-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae Rx1

92.157

100

0.922
  comGF/cglF Streptococcus pneumoniae D39

92.157

100

0.922
  comGF/cglF Streptococcus pneumoniae R6

92.157

100

0.922
  comGF/cglF Streptococcus pneumoniae TIGR4

92.157

100

0.922

Multiple sequence alignment