Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   SP4011_RS01260 Genome accession   NZ_AP026968
Coordinates   224992..226008 (+) Length   338 a.a.
NCBI ID   WP_338620380.1    Uniprot ID   -
Organism   Streptococcus parapneumoniae strain SP4011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219992..231008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP4011_RS01240 (SP4011_02210) nagA 221137..222288 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  SP4011_RS01245 (SP4011_02220) - 222451..223509 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  SP4011_RS01250 (SP4011_02230) - 223660..224025 (+) 366 WP_338619535.1 DUF1033 family protein -
  SP4011_RS01255 (SP4011_02240) comGA/cglA/cilD 224103..225044 (+) 942 WP_338619537.1 competence type IV pilus ATPase ComGA Machinery gene
  SP4011_RS01260 (SP4011_02250) comGB/cglB 224992..226008 (+) 1017 WP_338620380.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP4011_RS01265 (SP4011_02260) comGC/cglC 226010..226333 (+) 324 WP_338619538.1 competence type IV pilus major pilin ComGC Machinery gene
  SP4011_RS01270 (SP4011_02270) comGD/cglD 226296..226730 (+) 435 WP_044012294.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP4011_RS01275 (SP4011_02280) comGE/cglE 226693..226995 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP4011_RS01280 (SP4011_02290) comGF/cglF 226958..227419 (+) 462 WP_000250511.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP4011_RS01285 (SP4011_02300) comGG/cglG 227397..227810 (+) 414 WP_000265635.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP4011_RS01290 (SP4011_02310) - 228272..229114 (-) 843 WP_000840058.1 helix-turn-helix domain-containing protein -
  SP4011_RS01295 (SP4011_02320) - 229311..229787 (-) 477 WP_000777684.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38507.46 Da        Isoelectric Point: 9.8289

>NTDB_id=99078 SP4011_RS01260 WP_338620380.1 224992..226008(+) (comGB/cglB) [Streptococcus parapneumoniae strain SP4011]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMASLGFSSAI
VTQLSLAEVHGNLHLSLRKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVGVLLALTFYKRSSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLSQSLQNGREFSQTIGNYPFFRKELSLIIEYGEVKSKLGGELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=99078 SP4011_RS01260 WP_338620380.1 224992..226008(+) (comGB/cglB) [Streptococcus parapneumoniae strain SP4011]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGCACGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGCTAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGAGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATCATGCTGG
GACTACGGAACTATCTACTACCACAACTGGATAGTAGTAATATTGCCACTCAGATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGGTAGGATTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCCTAGCACGCATTCCTTTTCTTGGAATCTTTGTGCAGACTTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCTCAGGGAATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGTCTCAGTCCCTACAAAATGGTCGTGAATTTTCTCAGACGATAGGAAACTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATCGAGTATGGAGAAGTCAAGTCCAAGCTGGGTGGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

96.45

100

0.965
  comGB/cglB Streptococcus mitis SK321

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae Rx1

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae D39

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae R6

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae TIGR4

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

72.024

99.408

0.716
  comYB Streptococcus mutans UA140

58.307

94.379

0.55
  comYB Streptococcus mutans UA159

58.307

94.379

0.55
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment