Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   SP4011_RS01275 Genome accession   NZ_AP026968
Coordinates   226693..226995 (+) Length   100 a.a.
NCBI ID   WP_000413380.1    Uniprot ID   -
Organism   Streptococcus parapneumoniae strain SP4011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 221693..231995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP4011_RS01245 (SP4011_02220) - 222451..223509 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  SP4011_RS01250 (SP4011_02230) - 223660..224025 (+) 366 WP_338619535.1 DUF1033 family protein -
  SP4011_RS01255 (SP4011_02240) comGA/cglA/cilD 224103..225044 (+) 942 WP_338619537.1 competence type IV pilus ATPase ComGA Machinery gene
  SP4011_RS01260 (SP4011_02250) comGB/cglB 224992..226008 (+) 1017 WP_338620380.1 competence type IV pilus assembly protein ComGB Machinery gene
  SP4011_RS01265 (SP4011_02260) comGC/cglC 226010..226333 (+) 324 WP_338619538.1 competence type IV pilus major pilin ComGC Machinery gene
  SP4011_RS01270 (SP4011_02270) comGD/cglD 226296..226730 (+) 435 WP_044012294.1 competence type IV pilus minor pilin ComGD Machinery gene
  SP4011_RS01275 (SP4011_02280) comGE/cglE 226693..226995 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  SP4011_RS01280 (SP4011_02290) comGF/cglF 226958..227419 (+) 462 WP_000250511.1 competence type IV pilus minor pilin ComGF Machinery gene
  SP4011_RS01285 (SP4011_02300) comGG/cglG 227397..227810 (+) 414 WP_000265635.1 competence type IV pilus minor pilin ComGG Machinery gene
  SP4011_RS01290 (SP4011_02310) - 228272..229114 (-) 843 WP_000840058.1 helix-turn-helix domain-containing protein -
  SP4011_RS01295 (SP4011_02320) - 229311..229787 (-) 477 WP_000777684.1 GNAT family N-acetyltransferase -
  SP4011_RS01300 (SP4011_02330) - 229900..231102 (-) 1203 WP_000855226.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11135.11 Da        Isoelectric Point: 9.7273

>NTDB_id=99081 SP4011_RS01275 WP_000413380.1 226693..226995(+) (comGE/cglE) [Streptococcus parapneumoniae strain SP4011]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVAKMALQTGQNQVNINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=99081 SP4011_RS01275 WP_000413380.1 226693..226995(+) (comGE/cglE) [Streptococcus parapneumoniae strain SP4011]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTAATTTTACTGGAAGCAGTAGTCGCTCTAGCTATCTT
TGCCAGCATTGCGACCCTCCTTTTGGGACAAATTCAAAAAAATAGACAAGAAGAAGCAAAAATCTTGCAAAAGGAAGAAG
TCTTGAGGGTAGCTAAGATGGCCTTGCAGACAGGTCAAAATCAGGTAAACATAAATGGAGTTGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTTTATCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis NCTC 12261

99

100

0.99
  comGE/cglE Streptococcus pneumoniae Rx1

99

100

0.99
  comGE/cglE Streptococcus pneumoniae D39

99

100

0.99
  comGE/cglE Streptococcus pneumoniae R6

99

100

0.99
  comGE/cglE Streptococcus pneumoniae TIGR4

99

100

0.99
  comGE/cglE Streptococcus mitis SK321

98

100

0.98
  comYE Streptococcus mutans UA140

43.333

90

0.39
  comYE Streptococcus mutans UA159

43.333

90

0.39

Multiple sequence alignment