Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAE485_RS05670 Genome accession   NZ_CP151686
Coordinates   1109064..1110194 (+) Length   376 a.a.
NCBI ID   WP_215848935.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain QBS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1104064..1115194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAE485_RS05635 (AAE485_05550) - 1104201..1104770 (+) 570 WP_064219964.1 acyloxyacyl hydrolase -
  AAE485_RS05640 (AAE485_05555) bioB 1104812..1105762 (-) 951 WP_215861233.1 biotin synthase BioB -
  AAE485_RS05645 (AAE485_05560) - 1105759..1106109 (-) 351 WP_064219962.1 DUF167 domain-containing protein -
  AAE485_RS05650 (AAE485_05565) - 1106134..1106412 (-) 279 WP_215859736.1 YggT family protein -
  AAE485_RS05655 (AAE485_05570) proC 1106409..1107236 (-) 828 WP_409409713.1 pyrroline-5-carboxylate reductase -
  AAE485_RS05660 (AAE485_05575) - 1107233..1107904 (-) 672 WP_215880126.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAE485_RS05665 (AAE485_05580) pilT 1108005..1109054 (+) 1050 WP_064219958.1 type IV pilus twitching motility protein PilT Machinery gene
  AAE485_RS05670 (AAE485_05585) pilU 1109064..1110194 (+) 1131 WP_215848935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAE485_RS05675 (AAE485_05590) - 1110176..1111363 (+) 1188 WP_409409714.1 glycosyltransferase -
  AAE485_RS05680 (AAE485_05595) - 1111552..1111882 (-) 331 Protein_1118 type II toxin-antitoxin system YhaV family toxin -
  AAE485_RS05685 (AAE485_05600) - 1112026..1113309 (-) 1284 WP_409409715.1 hypothetical protein -
  AAE485_RS05690 (AAE485_05605) trmB 1113825..1114508 (-) 684 WP_226834373.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  AAE485_RS05695 (AAE485_05610) purE 1114559..1115056 (+) 498 WP_215849030.1 5-(carboxyamino)imidazole ribonucleotide mutase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41649.06 Da        Isoelectric Point: 5.7767

>NTDB_id=979821 AAE485_RS05670 WP_215848935.1 1109064..1110194(+) (pilU) [Acidithiobacillus ferriphilus strain QBS3]
MSVLDDLLVLMVEKNGSDLYLTVGSPPVMKIDGRAVSLGTEMLKPSQVLNLAKEILGMERLQEFQREKEINMAISAPGIG
RFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKDLAMTSRGLVLFVGATGSGKSTSLASMIQYRNQNAAGHILTIE
DPIEFLHKNAMSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLRAVVSQRLLPIKGQGGRVAAIEVLINTPAIADLIYKGEVGLLKDAMARTNDVGMQTFDQ
SLRQLFMDGLVEYEDALRGADSQNDLRLAIKQECMRRGVEDPGASTSAEGQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=979821 AAE485_RS05670 WP_215848935.1 1109064..1110194(+) (pilU) [Acidithiobacillus ferriphilus strain QBS3]
ATGTCTGTGCTGGATGATCTGCTGGTCTTGATGGTGGAAAAAAATGGCTCTGACCTTTACCTCACCGTAGGCTCGCCGCC
GGTCATGAAAATTGACGGGAGGGCGGTTTCGTTAGGGACCGAGATGCTGAAGCCGAGTCAGGTGCTGAATCTGGCCAAAG
AAATTCTGGGCATGGAGCGCTTGCAGGAATTCCAGCGGGAAAAAGAAATTAATATGGCCATCTCTGCGCCCGGGATTGGG
CGTTTCCGGGTCAATGGCTTTTTTCAGCGCGGTGAACTGAGCTTTGTCTTGCGCGCCATCAAGACGCAAATTCCCAGCCT
GGAACAGCTCAGGCTGCCGCCCATACTGAAAGACTTGGCCATGACTTCCCGAGGTCTGGTGCTCTTCGTCGGGGCTACCG
GCTCTGGTAAAAGTACCTCTCTGGCGTCGATGATTCAGTATCGCAATCAGAATGCGGCGGGCCATATTCTCACGATTGAG
GACCCCATCGAATTTCTTCACAAGAATGCGATGTCTATCGTCAATCAACGCGAAGTGGGTATCGATACCCTGAATTACGA
GCGGGCGCTGGAGAACGCCTTGCGTGAGGCGCCAGATGTTATTCTGATCGGCGAAGTGCGCAGTCGTGATACCATGGATC
ATGCCATGGCGTATGCAGAAACCGGACACTTGTGTATGTCGACCTTACATGCGAACAATGCTAATCAGGCCATTGAACGT
ATCATCAATTTTTTCCCGGAGGACCGTAAAAAACAGTTGTTGATGGATCTTTCACTCAATTTGCGCGCCGTGGTCTCACA
ACGCCTGTTGCCGATCAAGGGGCAGGGCGGGCGCGTCGCCGCTATAGAGGTGCTGATCAATACCCCGGCCATTGCCGATT
TGATCTATAAGGGTGAAGTGGGCTTGCTGAAAGACGCCATGGCGCGGACGAATGATGTGGGTATGCAGACGTTTGATCAG
AGCTTGCGACAACTGTTTATGGATGGGCTGGTGGAATATGAAGACGCCCTGCGCGGCGCCGATTCGCAGAATGACCTGCG
CCTTGCCATCAAGCAGGAGTGCATGCGGCGTGGCGTAGAGGACCCTGGTGCCAGTACTTCTGCAGAAGGGCAGTGGCGCA
TTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.345

92.553

0.559
  pilU Vibrio cholerae strain A1552

52.893

96.543

0.511
  pilU Acinetobacter baylyi ADP1

54.441

92.819

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.228

92.287

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.848

87.766

0.394
  pilT Vibrio cholerae strain A1552

44.848

87.766

0.394
  pilT Legionella pneumophila strain Lp02

42.09

89.096

0.375
  pilT Legionella pneumophila strain ERS1305867

42.09

89.096

0.375
  pilT Acinetobacter baylyi ADP1

42.342

88.564

0.375
  pilT Acinetobacter baumannii D1279779

40.653

89.628

0.364
  pilT Acinetobacter baumannii strain A118

40.653

89.628

0.364
  pilT Acinetobacter nosocomialis M2

40.653

89.628

0.364
  pilT Pseudomonas aeruginosa PAK

41.141

88.564

0.364
  pilT Neisseria meningitidis 8013

39.535

91.489

0.362

Multiple sequence alignment