Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   AABC73_RS24270 Genome accession   NZ_CP151076
Coordinates   5235965..5236372 (-) Length   135 a.a.
NCBI ID   WP_341521288.1    Uniprot ID   -
Organism   Pseudomonas sp. G.S.17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5230965..5241372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABC73_RS24240 (AABC73_24250) - 5231618..5232904 (+) 1287 WP_341521283.1 hypothetical protein -
  AABC73_RS24245 (AABC73_24255) - 5232901..5234004 (-) 1104 WP_341521284.1 cellulase family glycosylhydrolase -
  AABC73_RS24250 (AABC73_24260) - 5234107..5234874 (+) 768 WP_341521285.1 WecB/TagA/CpsF family glycosyltransferase -
  AABC73_RS24255 (AABC73_24265) - 5235087..5235380 (+) 294 WP_341521286.1 hypothetical protein -
  AABC73_RS24260 (AABC73_24270) - 5235431..5235703 (+) 273 WP_341521287.1 hypothetical protein -
  AABC73_RS24270 (AABC73_24280) pilA/pilA1 5235965..5236372 (-) 408 WP_341521288.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AABC73_RS24275 (AABC73_24285) pilB 5236597..5238294 (+) 1698 WP_341521289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABC73_RS24280 (AABC73_24290) pilC 5238297..5239514 (+) 1218 WP_341521290.1 type II secretion system F family protein Machinery gene
  AABC73_RS24285 (AABC73_24295) pilD 5239517..5240389 (+) 873 WP_341521291.1 A24 family peptidase Machinery gene
  AABC73_RS24290 (AABC73_24300) coaE 5240386..5241009 (+) 624 WP_341521292.1 dephospho-CoA kinase -
  AABC73_RS24295 (AABC73_24305) yacG 5241006..5241209 (+) 204 WP_065836197.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13596.81 Da        Isoelectric Point: 6.1132

>NTDB_id=974698 AABC73_RS24270 WP_341521288.1 5235965..5236372(-) (pilA/pilA1) [Pseudomonas sp. G.S.17]
MKAQKGFTLIELMIVIAIIGILAAIALPAYQDYTARAKASELILAASGARTCVTEAVSTNGFTGLAACGTDFVPTTYATT
LAVDAATGAIVVDGTINAAAVKVTLTPTVAATNANAIASWTCVGTPANWMPASCK

Nucleotide


Download         Length: 408 bp        

>NTDB_id=974698 AABC73_RS24270 WP_341521288.1 5235965..5236372(-) (pilA/pilA1) [Pseudomonas sp. G.S.17]
ATGAAGGCACAAAAAGGCTTTACCCTTATCGAACTGATGATCGTGATTGCGATTATCGGGATTTTGGCAGCGATTGCGTT
GCCCGCTTATCAGGACTACACCGCTCGCGCTAAAGCGTCAGAGCTGATCCTTGCTGCGAGCGGTGCGCGCACTTGCGTAA
CTGAGGCTGTGTCTACCAACGGTTTCACCGGCCTTGCTGCTTGTGGTACTGATTTTGTACCGACAACCTATGCGACAACA
CTTGCAGTGGATGCAGCAACCGGTGCAATCGTAGTAGACGGCACTATCAACGCCGCAGCGGTAAAGGTTACGTTGACTCC
GACAGTAGCGGCAACCAACGCCAACGCTATTGCCTCTTGGACATGCGTCGGCACACCAGCAAACTGGATGCCTGCTTCCT
GTAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

43.478

100

0.519
  comP Acinetobacter baylyi ADP1

43.333

100

0.481
  pilA2 Legionella pneumophila strain ERS1305867

44.526

100

0.452
  pilA2 Legionella pneumophila str. Paris

44.526

100

0.452
  pilA Haemophilus influenzae Rd KW20

42.143

100

0.437
  pilA Haemophilus influenzae 86-028NP

42.143

100

0.437
  pilA Ralstonia pseudosolanacearum GMI1000

42.748

97.037

0.415
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

100

0.415
  pilA Acinetobacter baumannii strain A118

38.732

100

0.407
  pilA Glaesserella parasuis strain SC1401

37.41

100

0.385
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

65.789

56.296

0.37

Multiple sequence alignment