Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AABC73_RS24275 Genome accession   NZ_CP151076
Coordinates   5236597..5238294 (+) Length   565 a.a.
NCBI ID   WP_341521289.1    Uniprot ID   -
Organism   Pseudomonas sp. G.S.17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5231597..5243294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABC73_RS24240 (AABC73_24250) - 5231618..5232904 (+) 1287 WP_341521283.1 hypothetical protein -
  AABC73_RS24245 (AABC73_24255) - 5232901..5234004 (-) 1104 WP_341521284.1 cellulase family glycosylhydrolase -
  AABC73_RS24250 (AABC73_24260) - 5234107..5234874 (+) 768 WP_341521285.1 WecB/TagA/CpsF family glycosyltransferase -
  AABC73_RS24255 (AABC73_24265) - 5235087..5235380 (+) 294 WP_341521286.1 hypothetical protein -
  AABC73_RS24260 (AABC73_24270) - 5235431..5235703 (+) 273 WP_341521287.1 hypothetical protein -
  AABC73_RS24270 (AABC73_24280) pilA/pilA1 5235965..5236372 (-) 408 WP_341521288.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AABC73_RS24275 (AABC73_24285) pilB 5236597..5238294 (+) 1698 WP_341521289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABC73_RS24280 (AABC73_24290) pilC 5238297..5239514 (+) 1218 WP_341521290.1 type II secretion system F family protein Machinery gene
  AABC73_RS24285 (AABC73_24295) pilD 5239517..5240389 (+) 873 WP_341521291.1 A24 family peptidase Machinery gene
  AABC73_RS24290 (AABC73_24300) coaE 5240386..5241009 (+) 624 WP_341521292.1 dephospho-CoA kinase -
  AABC73_RS24295 (AABC73_24305) yacG 5241006..5241209 (+) 204 WP_065836197.1 DNA gyrase inhibitor YacG -
  AABC73_RS24300 (AABC73_24310) - 5241232..5241927 (-) 696 WP_341521293.1 energy-coupling factor ABC transporter permease -
  AABC73_RS24305 (AABC73_24315) - 5241924..5242391 (-) 468 WP_065836195.1 hypothetical protein -
  AABC73_RS24310 (AABC73_24320) - 5242633..5243262 (+) 630 WP_020291059.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62600.06 Da        Isoelectric Point: 6.5162

>NTDB_id=974699 AABC73_RS24275 WP_341521289.1 5236597..5238294(+) (pilB) [Pseudomonas sp. G.S.17]
MSDVVLTGLAKQLVVAELLNEKAAQQAFQQARRDKVSLVSYLVQNKLVKALVLAEMASDQFGIPFFDLRSLDKDSQPKGL
VSEKLIRQHQALPLWRRGNKLFVGISDPSNHQAITDIQFNTGLTTEAILVEDDKLSDAIEKFFDSNSGMGDLADVDLGLE
IETVDDSKESTLSTKGDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKMFRVRLRTDGILHEVARPPIHLASRIAARLK
VMASLDISERRKPQDGRIKLRISKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALRQPQGM
ILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLE
TAEIAIKASQTGHLVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPHCKKEADIPRETLIKEGFPVD
QIGKFKIYAPVGCDLCNGGYKGRVGIYEVVKKTPALERIIMEEGNSLDISIQMRKDGFNDLRTSGLRKAMQGITSLEEVN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=974699 AABC73_RS24275 WP_341521289.1 5236597..5238294(+) (pilB) [Pseudomonas sp. G.S.17]
ATGAGTGATGTCGTCCTCACCGGTTTGGCCAAACAATTGGTTGTCGCCGAGCTTCTGAATGAAAAAGCTGCGCAGCAGGC
GTTTCAGCAAGCGCGCCGTGACAAGGTTTCCTTGGTCAGTTATCTGGTCCAGAACAAGCTGGTCAAGGCGCTTGTCCTCG
CAGAAATGGCGTCCGATCAATTTGGTATCCCGTTTTTTGACCTGAGAAGCCTGGACAAGGACAGCCAGCCCAAAGGCTTG
GTCAGCGAAAAACTCATTCGCCAGCATCAGGCCCTGCCTTTGTGGCGACGTGGCAACAAATTGTTCGTCGGAATTTCTGA
CCCCTCCAATCATCAAGCCATCACTGATATTCAATTCAATACCGGGCTCACTACCGAGGCCATTCTGGTCGAGGACGATA
AGCTCAGCGACGCTATCGAGAAGTTTTTCGATTCCAATAGCGGGATGGGCGATCTGGCAGATGTCGACCTCGGCCTGGAA
ATCGAAACAGTTGATGACAGTAAAGAAAGCACTCTGTCCACCAAGGGCGATGCGGACGATGCGCCAGTGGTGCGGTTCGT
CAACAAAATGTTGCTGGATGCCATCCGCCTCGGTTCATCTGACTTGCACTTTGAACCCTACGAAAAAATGTTCCGGGTGC
GTTTGCGTACCGATGGCATCCTGCACGAAGTCGCCAGGCCGCCGATTCATCTCGCCAGCCGCATCGCTGCGCGCCTGAAG
GTTATGGCTAGCCTGGATATTTCCGAACGACGCAAGCCTCAGGACGGGCGGATCAAGCTGCGCATCTCGAAAACCAAAGC
GATTGATTTCCGGGTCAATACCCTGCCGACGTTGTGGGGCGAAAAGATCGTAATGCGGATTCTTGATCCTACCAGTGCGC
AGATGGGTATCGACGCGTTGGGTTATGAACCAGATCAAAAAGAACTGTATCTGGAAGCATTGCGCCAGCCTCAGGGCATG
ATCCTCGTTACCGGCCCCACCGGTTCGGGTAAGACCGTATCGCTGTATACCGGCTTGAATATTCTCAACACCGTGGACAT
CAATATTTCCACGGCCGAAGACCCGGTCGAGATCAACCTTGAAGGCATAAACCAGGTCAACGTCAATCCGCGTCAAGGAA
TGGACTTTTCCCAAGCGCTGCGGGCCTTCCTGCGTCAGGATCCTGACGTGATCATGGTCGGCGAGATTCGTGACCTTGAA
ACCGCTGAAATTGCCATCAAGGCTTCGCAGACCGGTCACCTGGTATTGTCGACCCTGCACACCAACAGTGCTGCGGAAAC
GCTGACTCGCTTGCACCATATGGGCGTTGCGGCATTTAACATTGCCACGGCTATTAACCTGATCATTGCCCAGCGGTTGG
CGCGCAAGTTGTGTCCGCATTGCAAGAAAGAAGCGGATATCCCCCGGGAAACCCTGATAAAGGAAGGTTTCCCGGTAGAC
CAGATTGGCAAATTCAAGATTTACGCGCCGGTTGGGTGTGATTTATGCAACGGCGGTTACAAAGGCCGCGTCGGGATCTA
TGAGGTGGTCAAGAAAACCCCGGCGCTGGAACGGATCATCATGGAAGAAGGCAACTCCCTGGATATTTCCATCCAGATGC
GCAAAGACGGCTTCAATGACCTGCGCACATCGGGCCTGAGGAAGGCCATGCAAGGCATAACCAGCCTCGAAGAAGTCAAC
CGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.007

99.469

0.577
  pilB Acinetobacter baylyi ADP1

57.016

99.646

0.568
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.554
  pilB Vibrio cholerae strain A1552

50.709

99.823

0.506
  pilB Vibrio campbellii strain DS40M4

49.387

100

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

50.358

98.761

0.497
  pilF Neisseria gonorrhoeae MS11

48.135

99.646

0.48
  pilF Thermus thermophilus HB27

38.061

98.584

0.375

Multiple sequence alignment