Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WKW49_RS01025 Genome accession   NZ_CP149819
Coordinates   250479..251621 (+) Length   380 a.a.
NCBI ID   WP_015819970.1    Uniprot ID   C5BLK4
Organism   Teredinibacter turnerae strain SR02026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245479..256621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKW49_RS00995 (WKW49_00995) - 245598..246749 (-) 1152 WP_015817511.1 homoserine O-acetyltransferase -
  WKW49_RS01000 (WKW49_01000) - 246758..247057 (-) 300 WP_015816900.1 DUF167 family protein -
  WKW49_RS01005 (WKW49_01005) - 247120..247707 (-) 588 WP_015818511.1 YggT family protein -
  WKW49_RS01010 (WKW49_01010) proC 247781..248614 (-) 834 WP_339074845.1 pyrroline-5-carboxylate reductase -
  WKW49_RS01015 (WKW49_01015) - 248664..249350 (-) 687 WP_339074846.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKW49_RS01020 (WKW49_01020) pilT 249396..250430 (+) 1035 WP_015819744.1 type IV pilus twitching motility protein PilT Machinery gene
  WKW49_RS01025 (WKW49_01025) pilU 250479..251621 (+) 1143 WP_015819970.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WKW49_RS01030 (WKW49_01030) ruvX 251660..252100 (-) 441 WP_015820484.1 Holliday junction resolvase RuvX -
  WKW49_RS01035 (WKW49_01035) - 252093..252671 (-) 579 WP_015819114.1 YqgE/AlgH family protein -
  WKW49_RS01040 (WKW49_01040) - 252755..253636 (-) 882 WP_041590003.1 energy transducer TonB -
  WKW49_RS01045 (WKW49_01045) gshB 253636..254595 (-) 960 WP_339066943.1 glutathione synthase -
  WKW49_RS01050 (WKW49_01050) pilG 254842..255237 (+) 396 WP_015818072.1 twitching motility response regulator PilG Regulator
  WKW49_RS01055 (WKW49_01055) pilH 255283..255645 (+) 363 WP_015818447.1 twitching motility response regulator PilH -
  WKW49_RS01060 (WKW49_01060) - 255690..256229 (+) 540 WP_015817751.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42477.62 Da        Isoelectric Point: 6.3216

>NTDB_id=961703 WKW49_RS01025 WP_015819970.1 250479..251621(+) (pilU) [Teredinibacter turnerae strain SR02026]
MDFDRLLALMVEKGASDLFITAGVPPSMKLHGKVVPVSSTPLSPEKSRETVLSVMNEKQRKEFLETKELNFAISARGIGR
FRASAFYQRNLAGMVLRRIETQIPKIDDLGLPETIKELAMVKRGLVLFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCVITQREVGIDTESFEVALKNTLRQAPDVILIGEVRARETMDHAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLRAIVAQQLIPTPDGQGRRACLEIFLNTPLAQDLIRKGEVHELKELMKKSNEQGMQTFDQA
LYELYDAGEITYEDALSHADSPNDLRLMIKLGSESDAEYLKNAADELSIQEDDSHRTRKF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=961703 WKW49_RS01025 WP_015819970.1 250479..251621(+) (pilU) [Teredinibacter turnerae strain SR02026]
ATGGATTTTGATCGCCTGCTGGCGTTGATGGTTGAAAAAGGTGCGTCGGATTTGTTTATTACGGCCGGTGTTCCGCCGTC
CATGAAATTACACGGTAAAGTGGTTCCCGTTTCGTCGACGCCACTGTCGCCGGAAAAGAGCCGCGAAACCGTGCTCAGTG
TGATGAACGAAAAGCAGCGCAAAGAGTTTTTGGAAACCAAGGAACTCAACTTCGCGATTAGTGCACGAGGAATCGGGCGG
TTTCGTGCCAGCGCCTTCTATCAGCGCAACCTGGCGGGTATGGTGTTGCGTCGAATCGAAACACAAATTCCAAAAATTGA
TGATCTGGGCCTACCGGAAACCATTAAAGAGCTGGCGATGGTCAAGCGCGGTTTGGTGCTGTTTGTGGGGGCGACCGGTA
CTGGTAAGTCCACCTCTCTCGCCGCGATGATCGGTCACCGCAATCGCAACTCCAAAGGGCATATTATTTCCATTGAAGAC
CCTATCGAATTTATTCATCAGCATCAGGGTTGTGTTATCACTCAACGCGAAGTCGGTATTGATACGGAATCCTTTGAAGT
CGCGCTGAAAAATACTTTGCGACAAGCACCAGACGTCATTCTGATTGGTGAGGTGCGCGCTCGTGAAACCATGGATCACG
CGATTGCGTTTGCGGAAACCGGACATCTCTGTCTGGCGACCTTGCATGCGAACAATGCGAACCAGGCCTTGGACCGTATT
ATTCACTTCTTTCCGGCCGACCGTCACAGCCAGCTATGGATGGATTTGTCGTTAAACCTGCGAGCGATCGTTGCCCAGCA
GCTGATTCCTACCCCAGACGGGCAAGGGCGGCGTGCGTGTTTGGAGATTTTCTTAAATACGCCACTCGCTCAGGATTTAA
TTCGCAAAGGCGAAGTGCACGAGCTGAAAGAGCTGATGAAAAAATCCAACGAGCAGGGAATGCAGACGTTCGATCAGGCG
TTGTACGAACTTTATGACGCAGGCGAAATTACCTATGAAGATGCGCTTTCGCACGCGGATTCGCCGAACGATTTGCGCTT
GATGATTAAACTGGGCTCAGAGAGTGATGCCGAATACCTGAAAAATGCGGCCGATGAACTGAGTATTCAGGAAGATGATT
CGCATCGCACTCGCAAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(38-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C5BLK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

77.895

100

0.779
  pilU Acinetobacter baylyi ADP1

66.947

93.947

0.629
  pilU Vibrio cholerae strain A1552

55.429

92.105

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.638

90.789

0.405
  pilT Legionella pneumophila strain Lp02

42.687

88.158

0.376
  pilT Legionella pneumophila strain ERS1305867

42.687

88.158

0.376
  pilT Pseudomonas aeruginosa PAK

40.58

90.789

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.95

88.684

0.363
  pilT Vibrio cholerae strain A1552

40.95

88.684

0.363
  pilT Acinetobacter nosocomialis M2

40.351

90

0.363
  pilT Acinetobacter baumannii D1279779

40.351

90

0.363
  pilT Acinetobacter baumannii strain A118

40.351

90

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.71

90.789

0.361